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Status |
Public on Nov 24, 2009 |
Title |
Drosophila pseudoobscura and Drosophila simulans small RNAs |
Organisms |
Drosophila pseudoobscura; Drosophila simulans |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
High-throughput sequencing of Drosophila pseudoobscura and Drosophila simulans small RNAs. ~18-26nt RNAs were isolated from total RNA using PAGE, ligation to adapters requires 5' monophosphate and 3' OH.
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Overall design |
Small RNAs were cloned from Drosophila pseudoobscura (heads and pooled 0-12 and 12-24 hour embryos) and Drosophila simulans (pooled 0-12 and 12-24 hour embryos). Sequencing was performed using the Illumina 1G platform. Following removal of 3' linker sequences, the clipped sequences longer than 18 nt were kept.
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Contributor(s) |
Berezikov E, Liu N, Flynt AS, Lai EC |
Citation(s) |
20037610 |
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Submission date |
Nov 19, 2008 |
Last update date |
May 15, 2019 |
Contact name |
Eugene Berezikov |
E-mail(s) |
e.berezikov@umcg.nl
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Phone |
+31 50 361 73 00
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Organization name |
University Medical Center Groningen
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Department |
European Research Institute for the Biology of Ageing
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Street address |
Antonius Deusinglaan 1
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City |
Groningen |
ZIP/Postal code |
9713AV |
Country |
Netherlands |
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Platforms (2) |
GPL9394 |
Illumina Genome Analyzer (Drosophila simulans) |
GPL9395 |
Illumina Genome Analyzer (Drosophila pseudoobscura) |
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Samples (3) |
GSM343915 |
total small RNAs from embryos (Drosophila simulans) |
GSM343916 |
total small RNAs from embryos (Drosophila pseudoobscura) |
GSM444067 |
total small RNAs from heads (Drosophila pseudoobscura) |
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Relations |
SRA |
SRP001579 |
BioProject |
PRJNA110425 |