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Series GSE136789 Query DataSets for GSE136789
Status Public on Sep 16, 2019
Title Oligomeric human Aβ1-42 inhibits antigen presentation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study is to investigate the involvement of inflammation in Alzheimer’s disease (AD) and to clarify the signaling pathways involved in the presence of beta-amyloidosis, a hallmark of AD pathogenesis, to help identifying potential targets for therapy. To do that, we isolated bone marrow-derived progenitor cells from femurs, tibiae and hip bones of non-transgenic C57BL/6 mice according to established protocols , and we maturated them with LPS. To obtain an unbiased view of gene regulation in mouse bone marrow-derived dendritic cells (BM-DCs) exposed to pre-aggregated beta-amyloid peptide (Aβ) oligomers, we analyzed the transcriptome of untreated immature control BM-DCs (‘Ctrl’), LPS-treated BM-DCs (’LPS’), Aβ1-42 oligomer-treated BM-DCs (‘Aβ‘) and BM-DCs treated with Aβ1-42 oligomers and LPS (‘Aβ+LPS‘) via explorative RNA-sequencing.
 
Overall design Relative comparison of mRNA profile of mouse bone marrow-derived dendritic cells with different treatments: (1) control bone marrow-derived dendritic cells (‘Ctrl’), (2) LPS-treated bone marrow-derived dendritic cells (’LPS’), (3) Aβ1-42 oligomer-treated bone marrow-derived dendritic cells (‘Aβ‘), (4) and bone marrow-derived dendritic cells treated with Aβ1-42 oligomers and LPS (‘Aβ+LPS‘). The RNA sequencing was performed in single reads of 100bp length using Illumina HiSeq3000 (Illumina), data were generated in triplicate.
 
Contributor(s) Gericke C, Mallone A, Engelhardt B, Nitsch RM, Ferretti MT
Citation(s) 32582162
Submission date Sep 03, 2019
Last update date Jun 29, 2020
Contact name Anna Mallone
E-mail(s) anna.mallone@uzh.ch
Organization name University of Zurich
Department Institute for Regenerative Medicine
Street address Wagistrasse 12
City Schlieren
State/province Zurich
ZIP/Postal code 8952
Country Switzerland
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM4057932 Untreated_control_rep1
GSM4057933 Untreated_control_rep2
GSM4057934 Untreated_control_rep3
Relations
BioProject PRJNA563674
SRA SRP220156

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Supplementary file Size Download File type/resource
GSE136789_Abeta_trtm.LPS_Abeta_trtm.DEResults.xlsx 4.4 Mb (ftp)(http) XLSX
GSE136789_Abeta_trtm.LPS_ctrl.DEResults.xlsx 6.0 Mb (ftp)(http) XLSX
GSE136789_Abeta_trtm.ctrl.DEResults.xlsx 6.0 Mb (ftp)(http) XLSX
GSE136789_LPS_Abeta_trtm.LPS_ctrl.DEResults.xlsx 4.6 Mb (ftp)(http) XLSX
GSE136789_LPS_Abeta_trtm.ctrl.DEResults.xlsx 4.6 Mb (ftp)(http) XLSX
GSE136789_LPS_ctrl.ctrl.DEResults.xlsx 10.8 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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