|
| Status |
Public on Nov 21, 2008 |
| Title |
Regulation of lymphoid versus myeloid fate choice by Mef2c |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Despite recent advances in the identification of lymphoid-restricted progenitors, the transcription factors essential for their generation remain to be identified. Here we describe an unexpected role for the myeloid oncogene Mef2c in multipotent progenitors (MPPs), where it is required for pan-lymphoid differentiation. Mef2c deficiency was associated with profound defects in B, T, NK cell and common lymphoid progenitor production and an enhanced myeloid output. Mef2c deficiency in MPPs leads to downregulation of several key lymphoid regulators and the upregulation of the myeloid factor C/EBPa. Our studies also show that Mef2c is a critical transcriptional target of PU.1 during lymphopoiesis. Thus, Mef2c is a crucial component of the transcriptional network that regulates lymphoid specification and cell fate choice in MPPs.
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| Overall design |
Expression profiles of multipotent progenitor cells (MPPs) from Mef2c deficient (Mx1-Cre Mef2cf/f ) mice where compared to MPPs from control (Mef2cf/f ) mice in triplicate.
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| Contributor(s) |
Stehling-Sun S, Camargo FD |
| Citation(s) |
19169261 |
| |
| Submission date |
Nov 20, 2008 |
| Last update date |
May 10, 2018 |
| Contact name |
Fernando Camargo |
| E-mail(s) |
camargo@wi.mit.edu
|
| Organization name |
Whitehead Institute
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
|
| Samples (3) |
| GSM343288 |
Expression profiling on Mef2c deficient multipotent progenitor cells Replicate1 |
| GSM343289 |
Expression profiling on Mef2c deficient multipotent progenitor cells Replicate2 |
| GSM343290 |
Expression profiling on Mef2c deficient multipotent progenitor cells Replicate3 |
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| Relations |
| BioProject |
PRJNA110441 |
