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Series GSE13688 Query DataSets for GSE13688
Status Public on Nov 20, 2009
Title Effect of TCPOBOP and PCN in combination with high-cholesterol diet on genes involved in cholesterol homeostasis
Organism Mus musculus
Experiment type Expression profiling by array
Summary TCPOBOP (1,4-bis[2-(3,5-dichloropyridyloxy)]benzene) and PCN (pregnenolone 16α-carbonitrile) are inducers of drug metabolism through activation of nuclear receptors CAR (constitutive androstane receptor) and PXR (pregnane X receptor), respectively. Mouse experiment was designed to study the effect of CAR and PXR activation on cholesterol homeostasis genes and other genes, which are present on the Steroltalk v2 microarray. Treatments were combined with standard and high-cholesterol diet to observe the interference of high liver cholesterol on nuclear receptor transcription regulation. All experiments were done within the European sixth Framework program “Steroltalk” ( Results form these experiments give new knowledge about involvement of ‘xenosensors’ CAR and PXR in regulation of endogenous liver metabolism.
Overall design Animals were injected i.p. 3mg/kg TCPOBOP, 40 mg/kg PCN or vehicle (corn oil) in combination of one week of standard or 1% cholesterol diet prior tretament. After 24h they were sacrificed and total RNA was isolated from the livers. Each sample was hybridized with a reference sample to Steroltalk v2 microarrays.
Contributor(s) Režen T, Rozman D
Citation(s) 19691840
Submission date Nov 20, 2008
Last update date Mar 20, 2012
Contact name Peter Juvan
Phone +386 1 543 7595
Organization name Faculty of Medicine, University of Ljubljana
Department Institute of Biochemistry
Lab Center for Functional Genomics and Bio-Chips
Street address Zaloska 4
City Ljubljana
ZIP/Postal code SI-1000
Country Slovenia
Platforms (1)
GPL7190 Steroltalk v2 mouse
Samples (42)
GSM344074 Liver_stand_control_1
GSM344075 Liver_stand_control_2
GSM344076 Liver_stand_control_3
This SubSeries is part of SuperSeries:
GSE13721 Effect of TCPOBOP, PCN, rosuvastatin, and atorvastatinin on cholesterol homeostasis
BioProject PRJNA114573

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13688_RAW.tar 3.6 Mb (http)(custom) TAR (of TAB, TXT)
Processed data included within Sample table

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