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| Status |
Public on Aug 04, 2020 |
| Title |
Comparison of tumor-associated YAP1 fusions identifies a recurrent set of functions critical for oncogenesis[RNASeq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Several different YAP1 gene fusions have been identified in various human cancers. We performed RNA sequencing on human neural stem cells overexpressing either YAP1-MAMLD1, YAP1-FAM118B, or YAP1-TFE3, as well as on whole tumor lysates from mouse brain tumors generated by intra-cranial expression of either YAP1-MAMLD1, YAP1-FAM118B, or YAP1-TFE3.
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| Overall design |
We performed RNA sequencing on human neural stem cells overexpressing either YAP1-MAMLD1, YAP1-FAM118B, or YAP1-TFE3. U5 huamn neural stem cells were transduced with pLJM1-"transgene"-PGK-Puro. 48 hrs post transduction, positive clones were selected by 72 hrs of Puromycin selection. Total RNA was extracted 48 hrs after Puromycin selection.
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| Contributor(s) |
Szulzewsky F, Holland EC, Arora S |
| Citation(s) |
32675324 |
| |
| Submission date |
Sep 06, 2019 |
| Last update date |
Aug 07, 2020 |
| Contact name |
Sonali Arora |
| E-mail(s) |
sarora@fredhutch.org
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| Organization name |
FHCRC
|
| Street address |
1100 Fairview Ave N,
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE137040 |
Comparison of tumor-associated YAP1 fusions identifies a recurrent set of functions critical for oncogenesis |
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| Relations |
| BioProject |
PRJNA564315 |
| SRA |
SRP220703 |
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