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Series GSE137224 Query DataSets for GSE137224
Status Public on Feb 25, 2021
Title Infection with Toxoplasma gondii induces inheritable epigenetic changes in mouse sperm
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In this study we sought to analyse if infection with the common human parasite Toxoplasma gondii aftered sperm epigenetics by sequecing small RNA from parasite infected and mock infected mice. Epigenetic changes in sperm is a well described process of inheritanece of paternal environmental experiences to their progeny. Using high throughput Illumina sequencing, we revealed differences between the sperm small RNA profiles of Toxoplasma-infected and control animals . We found 25.88% of small RNAs were differentially expressed after correcting for false discovery rate, of which 9.21% and 16.67% were up- and down-regulated, respectively. Further quantitative analysis revealed enriched small RNA load in our samples and biotypes composition analysis showed a majority tRNA population followed by miRNA and piRNA populations, while the rest contained various other small RNA transcripts.When we looked at miRNA changes between Toxoplasma-infected and control samples, we found that of the total miRNA mapped (585), 174 were differentially expressed in the Toxoplasma-infected group (FDR < 0.05) of which 35 were down- and 75 were up-regulated with a Log2FC < -2 and > 2, respectively. Lastly, using gene enrichment analysis, we identified pathways regulated by small RNA’s that are differentially expressed and found that small RNA’s targeted distinct biological pathways.
 
Overall design Sequencing of sperm small RNA from mice injected with PBS control or Toxoplasma. 5 biological replicates in each group, each consisting of samples pooled from 2 mice.
 
Contributor(s) Shiraz T, Christopher TJ
Citation(s) 32348768
Submission date Sep 10, 2019
Last update date Feb 25, 2021
Contact name Shiraz Tyebji
E-mail(s) shiraz86@gmail.com
Phone 0405285135
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Infectious diseases and immune defence division
Lab Tonkin Lab
Street address 1G Royal Parade
City Melbourne
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (10)
GSM4073578 Shiraz_1
GSM4073579 Shiraz_2
GSM4073580 Shiraz_3
Relations
BioProject PRJNA564827
SRA SRP221277

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Supplementary file Size Download File type/resource
GSE137224_CAGRF18125-Infected.vs.Control-all_genes.csv.gz 1.6 Mb (ftp)(http) CSV
GSE137224_features_summary.csv.gz 539 b (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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