|
| Status |
Public on Sep 13, 2019 |
| Title |
c-Maf regulates the plasticity of group 3 innate lymphoid cells by restraining the type 1 program [CUT&RUN] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The transcription factor c-Maf is an essential regulator of group 3 ILC homeostasis and effector plasticity. c-Maf limits acquisition of the type 1 program and conversion to the ILC1 fate through restraint of T-bet expression and function.
|
| |
|
| Overall design |
Evaluation of differential H3K27ac and H3K27me3 histone marks in cohoused Maf-deficient NKp46+ ILC3. The CUT&RUN protocol was performed on replicate samples of NKp46+ ILC3 isolated from the small intestine lamina propria of Maf+/+ Il7r-Cre and Maf fl/fl Il7r-Cre mice.
|
| |
|
| Contributor(s) |
Ciofani M, Parker ME, Barrera A |
| Citation(s) |
31570496 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM115474 |
The role of AP-1 family transcription factor networks in regulating Th17 cell effector identity |
DUKE UNIVERSITY |
Maria Ciofani |
|
| |
| Submission date |
Sep 12, 2019 |
| Last update date |
Dec 14, 2019 |
| Contact name |
Maria Ciofani |
| E-mail(s) |
maria.ciofani@duke.edu
|
| Organization name |
Duke University School of Medicine
|
| Department |
Integrative Immunobiology
|
| Street address |
207 Research Drive, 128 Jones Building
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (11)
|
| GSM4075364 |
IgG NKp46+ ILC3 Maf+/+ Il7rCre Rep 1 |
| GSM4075365 |
H3K27ac NKp46+ ILC3 Maf+/+ Il7rCre Rep 1 |
| GSM4075366 |
H3K27me3 NKp46+ ILC3 Maf+/+ Il7rCre Rep 1 |
| GSM4075367 |
IgG NKp46+ ILC3 Maf fl/fl Il7rCre Rep 1 |
| GSM4075368 |
H3K27ac NKp46+ ILC3 Maf fl/fl Il7rCre Rep 1 |
| GSM4075369 |
H3K27me3 NKp46+ ILC3 Maf fl/fl Il7rCre Rep 1 |
| GSM4075370 |
H3K27ac NKp46+ ILC3 Maf+/+ Il7rCre Rep 2 |
| GSM4075371 |
H3K27me3 NKp46+ ILC3 Maf+/+ Il7rCre Rep 2 |
| GSM4075372 |
IgG NKp46+ ILC3 Maf fl/fl Il7rCre Rep 2 |
| GSM4075373 |
H3K27ac NKp46+ ILC3 Maf fl/fl Il7rCre Rep 2 |
| GSM4075374 |
H3K27me3 NKp46+ ILC3 Maf fl/fl Il7rCre Rep 2 |
|
| This SubSeries is part of SuperSeries: |
| GSE137322 |
c-Maf regulates the plasticity of group 3 innate lymphoid cells by restraining the type 1 program |
|
| Relations |
| BioProject |
PRJNA565167 |
| SRA |
SRP221455 |
Less...
More...