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Series GSE137678 Query DataSets for GSE137678
Status Public on May 28, 2020
Title STAT5 is required for lipid breakdown and beta-adrenergic responsiveness of brown adipose tissue
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Increasing energy expenditure through activation of brown adipose tissue (BAT) thermogenesis is an attractive approach to counteract obesity. Thus, it is essential to understand molecular mechanisms that control BAT functions. Here, we describe signal transducer and activator of transcription (STAT) 5 as key regulator of BAT functionality. We found that STAT5 is necessary for acute cold-induced temperature maintenance and stimulated lipid breakdown in BAT using mice that harbour an adipocyte-specific deletion of Stat5a/b genes. In addition, the mitochondrial respiratory capacity of primary differentiated brown adipocytes from STAT5 deficient mice was diminished. We show that increased sensitivity to cold stress upon STAT5 deficiency was associated with reduced expression of thermogenic key player uncoupling protein 1, while decreased stimulated lipolysis of STAT5-deficient BAT explants was linked to decreased protein kinase A activity. In addition, brown remodeling of white fat was diminished following chronic β-adrenergic stimulation. This impairment was linked to a decrease in mitochondrial functionality. We conclude that STAT5 is essential for the β-adrenergic responsiveness of brown adipose tissue and the physiologic function of thermogenic adipose tissue.
 
Overall design Wildtype and Stat5Adipoq, 5 replicates per condition
Web link https://doi.org/10.1016/j.molmet.2020.101026
 
Contributor(s) Kaltenecker D, Spirk K, Ruge F, Grebien F, Rupprecht A, Kenner L, Pohl E, Mueller KM, Moriggl R
Citation(s) 32473405
Submission date Sep 18, 2019
Last update date Aug 27, 2020
Contact name Tobias Suske
E-mail(s) tobias.suske@vetmeduni.ac.at
Organization name University of Veterinary Medicine
Department Functional Cancer Genomics
Lab Moriggl
Street address Veterinärplatz 1
City Vienna
State/province Vienna
ZIP/Postal code 1210
Country Austria
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (10)
GSM4084087 control_basal_1
GSM4084088 control_basal_2
GSM4084089 control_basal_3
Relations
BioProject PRJNA566194
SRA SRP222364

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137678_DE_BOTH_45_control_basal_vs_Stat5Adipoq_basal_DE_genes_005.id.csv.gz 43.0 Kb (ftp)(http) CSV
GSE137678_DE_DOWN_45_control_basal_vs_Stat5Adipoq_basal_DE_genes_005.id.csv.gz 22.3 Kb (ftp)(http) CSV
GSE137678_DE_UP_45_control_basal_vs_Stat5Adipoq_basal_DE_genes_005.id.csv.gz 21.8 Kb (ftp)(http) CSV
GSE137678_GO_analyses_BAT_kaltenecker.xlsx 30.3 Kb (ftp)(http) XLSX
GSE137678_RAW.tar 870.0 Kb (http)(custom) TAR (of TXT)
GSE137678_res1_45_control_basal_vs_Stat5Adipoq_basal.id.csv.gz 830.8 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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