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Series GSE137792 Query DataSets for GSE137792
Status Public on Dec 10, 2019
Title Sex chromosomes affect gene expression of healthy CD4+ T cells in SJL.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To determine the role of sex chromsomes in sex differences of autoimmune disease, we used high throughput RNA sequencing to analyze the transciptomes of CD4+ T cells stimuated with anti-CD3/CD28 from XX and XY- mice using the “four core genotypes” mouse model. We found higher expression of several X genes in the XY- genotype compared to XX, suggesting a difference in DNA methylation of these genes between Xp and Xm. In a hypothesis driven targeted approach, we investigated two immunomodulatory X genes, Tlr7 and FoxP3 and found that the promoter regions of both genes had more DNA methylation on the Xp than Xm, and that both genes had higher expression in autoantigen stimulated CD4+ T cells from XY- compared to XX mice. This study provides new insights regarding how sex chromsomes affect gene expression and cause sex differences in autoimmune disease.
Overall design Female mice from the "four core genotypes" mouse model (XX and XY-) were gonadectomized at 4-6 weeks old. At 8-12 weeks old, auxillary, brachial, and inguinal draining lymph nodes were collected and processed into single cell suspensions and sorted for CD4+ T cells using the EasySep Mouse CD4+ T cell isolation kit (STEMCELL). Cells were stiumulated with anti-CD3/CD28 Dyna Beads for 36 hours and RNA was isolated for analysis by high throughput RNA sequencing.
Contributor(s) Golden L, Itoh Y, Itoh N, Voskuhl R
Citation(s) 31822606
Submission date Sep 20, 2019
Last update date Jan 08, 2020
Contact name Yuichiro Itoh
Phone 3102068999
Organization name UCLA
Department Neurology
Street address 635 Charles E. Young Drive South
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (12)
GSM4087907 Sample 1: XX1
GSM4087908 Sample 2: XX2
GSM4087909 Sample 3: XX3
BioProject PRJNA573078
SRA SRP222822

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Supplementary file Size Download File type/resource
GSE137792_SJLcd4FCGhealthy_rawcounts.xlsx 2.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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