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Series GSE13817 Query DataSets for GSE13817
Status Public on Aug 01, 2009
Title Deletion of Clr3 restores expression of a subset of genes in gcn5- mutant cells
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by array
Summary Histone acetylation and deacetylation is important for gene regulation. The histone acetyltransferase, Gcn5, is a known activator of transcriptional initiation that is recruited to gene promoters. Here we map genome-wide levels of Gcn5 occupancy and histone H3K14ac at high resolution. Gcn5 is predominantly localized to coding regions of highly transcribed genes where it antagonistically collaborates with the class II histone deacetylase, Clr3, to regulate histone H3K14ac levels. Regulation of histone H3k14ac levels is critical for regulation of many genes during stress adaptation. Our findings suggest a novel role for Gcn5 during transcriptional elongation in addition to its known role in transcriptional initiation.
The related data for this are GSE13790 and GSE5227
Overall design Data showed expression pattern of gcn5- vs gcn5-clr3- and clr3- vs wild type under KCl stress in S. pombe. Two biological replicates are used with dye-swap labeling.
Contributor(s) Johnsson A, Durand-Dubief M, Xue-Franzén Y, Ekwall K, Rönnerblad M, Wright A
Citation(s) 19633696
Submission date Dec 03, 2008
Last update date Mar 20, 2012
Contact name Anthony Wright
Organization name Karolinska Institute
Lab Anthony Wright
Street address Blickagången 6
City Huddinge
ZIP/Postal code 141 57
Country Sweden
Platforms (2)
GPL3425 Eurogentec AG S. pombe ORF array 11k (I100C)
GPL7734 Eurogentec AG S. pombe ORF array 11k (spD080C)
Samples (4)
GSM347638 Interplay between HDAC clr3 and HAT gcn5 replicate 2
GSM347643 Interplay between HDAC clr3 and HAT gcn5 replicate 1
GSM392030 Schizosaccharomyces pombe clr3 mutant compared to wt KCl treatment,replicate 1 (slide 2)
BioProject PRJNA110591

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