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Series GSE138199 Query DataSets for GSE138199
Status Public on Sep 10, 2020
Title Organism Dual RNA-Seq Reveals the Control on both Pathogen and Host through BarA/UvrY of Vibrio parahaemolyticus for Successful Infection
Organisms Vibrio parahaemolyticus; Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary Elucidation of host-pathogen interaction is essential for developing effective strategies to combat bacterial infection. Dual RNA-Seq using cultured cells or tissues/organs as the pathogen host has emerged as a novel strategy to understand the response concurrently from both pathogen and host at cellular level. However, bacterial infection mostly causes systematic responses from the host at organism level where the interplay is urgently to be understood but inevitably being neglected by the current practice. Here, we developed an approach that simultaneously monitor the genome-wide infection-linked transcriptional alterations in both pathogenic Vibrio parahaemolyticus and the infection host nematode Caenorhabditis elegans. Besides the dynamic alterations in both transcriptomes of C. elegans and V. parahaemolyticus during infection, we identify a two-component system, BarA/UvrY, that are essential for host colonization. BarA/UvrY not only controls the virulence factors in V. parahaemolyticus including Type III and Type VI secretion systems, but also represses the MAP kinase mediated signaling cascades to attenuate innate immune responses. Thus, our study exemplifies the use of dual RNA-Seq at organism level to uncover previously unrecognized interplay between host and pathogen.
Overall design Investagation of cross-talk between host and pathogen
Contributor(s) Xie R, Zheng J
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Submission date Sep 30, 2019
Last update date Sep 13, 2020
Contact name wenwen Zhang
Organization name Zhang Wenwen
Street address University of Macau, Avenida de Universidade, Taipa
City Macau
ZIP/Postal code no
Country Macau
Platforms (3)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
GPL19796 Illumina HiSeq 2500 (Vibrio parahaemolyticus)
GPL27555 Illumina HiSeq 2500 (Caenorhabditis elegans; Vibrio parahaemolyticus)
Samples (36)
GSM4101785 V12_1
GSM4101786 V12_2
GSM4101787 V12_3
BioProject PRJNA575028
SRA SRP223768

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Supplementary file Size Download File type/resource
GSE138199_RAW.tar 7.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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