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Series GSE138234 Query DataSets for GSE138234
Status Public on Nov 11, 2020
Title Gtf2i and Gtf2ird1 E13.5 whole brain ChIP-seq and RNA-seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary HetxHet breeding pairs for the Gtf2i/Gtf2ird1 double mutants and Gtf2ird1 were set up for timed breedings. E13.5 embyros of WT, HET, and HOM mutants (n=3 for each genotype and each cross), were used for RNA-seq. Similar breedings were done for ChIP-seq. The ChIP-seq WT controls were E13.5 embyros from WT FVB/ANTJ x FVB/ANTJ and compared to HOM Gtf2i/Gtf2ird1 double mutatns and HOM Gtf2ird1 single mutants. There were n=3 WT and n=3 hom Gtf2ird1 single mutants for Gtf2ird1 ChIP-seq. There were n=4 WT and n=4 hom Gtf2i/Gtf2ird1 double mutants for Gtf2i ChIP-seq. Each genotype had the sample matched input control along with ChIP sample.
 
Overall design Gtf2i and Gtf2ird1 E13.5 ChIP-seq and RNA-seq
 
Contributor(s) Kopp ND, Dougherty JD
Citation(s) 32313931
Submission date Oct 01, 2019
Last update date Apr 09, 2021
Contact name Joseph D Dougherty
Organization name Washington University
Department Genetics and Psychiatry
Street address 4566 Scott Ave, box 8232
City St Louis
State/province Missouri
ZIP/Postal code 63110
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (50)
GSM4103136 11610-12
GSM4103137 11610-3
GSM4103138 11610-1
Relations
BioProject PRJNA575176
SRA SRP223850

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Supplementary file Size Download File type/resource
GSE138234_Gtf2i_Gtf2ird1_RNA_seq.csv.gz 443.2 Kb (ftp)(http) CSV
GSE138234_MACS2_ouput_files.tar.gz 10.6 Mb (ftp)(http) TAR
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Raw data are available in SRA
Processed data are available on Series record

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