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Series GSE138271 Query DataSets for GSE138271
Status Public on Apr 01, 2022
Title Human Dental Pulp Cell exosomes vs Human iPS Cell exosomes
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Small vesicles, known as exosomes, are secreted from various cell types. Exosomes secreted by mesenchymal stem cells have therapeutic effects against a variety of diseases, and may be able to partially replace stem cell therapies. Previously, we established and characterized human leukocyte antigen (HLA) haplotype homo (HHH) dental pulp cell (DPC) lines from human wisdom teeth. In this report, we purified the exosomes secreted from HHH-DPCs and evaluated their therapeutic potential in a periodontitis model. The exosomes purified from HHH-DPCs showed homogeneous and spherical membrane structures, and showed low but significant expression of HLA class I molecules. The exosomes further promoted proliferation and migration in DPCs. A comparison of miRNAs revealed that the HHH-DPC exosomes contained higher levels of multiple Let-7 family miRNAs compared to HHH-induced pluripotent stem cell (iPSC)-derived exosomes. Finally, the HHH-DPC exosomes showed preventive effects in a mouse model of periodontitis induced by lipopolysaccharides (LPS). In summary, HHH-DPC exosomes expressed HLA molecules which may induce an immune response in HLA-mismatched transplantations. However, they successfully stimulated the proliferation and migration of cells and showed suppressive effects on LPS-induced periodontitis. Therefore, HHH-DPC exosomes show great potential for applications in periodontal treatments.
 
Overall design To identify genes expressed in exosomes, comprehensive gene expression analysis was performed using exosomes from DP74, DP94 and DP263 and exosomes from iPS74, iPS94 and iPS263.
 
Contributor(s) Tezuka K, Shimizu Y, Kawaguchi T
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Submission date Oct 01, 2019
Last update date Apr 02, 2022
Contact name Ken-ichi Tezuka
E-mail(s) tezuka@gifu-u.ac.jp
Phone +81-58-230-6476
Organization name Gifu University Graduate School of Medicine
Department Gifu University Graduate School of Medicine
Street address 1-1 Yanagido
City Gifu City
State/province Gifu
ZIP/Postal code 501-1194
Country Japan
 
Platforms (1)
GPL25134 Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version)
Samples (6)
GSM4104210 DP74 exosomes
GSM4104211 DP94 exosomes
GSM4104212 DP263 exosomes
Relations
BioProject PRJNA575247

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138271_RAW.tar 40.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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