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Series GSE138471 Query DataSets for GSE138471
Status Public on Nov 18, 2019
Title Detection of differences in gene expression in the noradrenergic nuclei in the brain
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study was to compare the transcriptome of neurons from the locus coeruleus (LC) from wild type and mice that are conditional KO to argininosuccinatelyase (ASL) in the LC. Using an optimized data analysis, we were able to detect difference in the transcription of key enzymes in the synthesis of catecholamine, along with changes in the catecholamine synthesis pathway. Approximately 7% of the transcripts showed differential expression between the WT and ASL-LC cKO mice. The altered expression of the releant genes was confirmed with qRT–PCR. Our study represents the detailed analysis of the LC that revealed novel metabolic regulation on this region.
 
Overall design 100 cells from each mouse were collected using laser micro dissection from the LC of WT and ASL-LC cKO mice
 
Contributor(s) Lerner S, Erez A
Citation(s) 31747589
Submission date Oct 06, 2019
Last update date Dec 31, 2019
Contact name Ayelet Erez
E-mail(s) ayelet.erez@weizmann.ac.il
Phone 972-8-934-3714
Organization name Weizmann Institute of Science
Department Biological Regulation
Street address 234 Herzl Street
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM4109500 LC, WT rep1
GSM4109501 LC, WT rep2
GSM4109502 LC, WT rep3
Relations
BioProject PRJNA576067
SRA SRP224526

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138471_processed1_raw_counts.xlsx 1.4 Mb (ftp)(http) XLSX
GSE138471_processed2_normalized_counts.xlsx 2.4 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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