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Series GSE138594 Query DataSets for GSE138594
Status Public on May 14, 2020
Title Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of the mesenteric lymph node II
Organism Mus musculus
Experiment type Expression profiling by array
Summary Obesity has emerged as a worldwide problem in human health. Dietary lipids are taken up and transported via lymphatics into the circulatory system. During this process, lipids pass the mesenteric lymph node (mLN). These organs filter the lymph fluid for foreign antigens to induce and control immune responses. Alteration of that function during obesity has only slightly been studied. Here, we characterize changes within the microarchitecture of the mLN during high levels of lipid transport and highlight the role of stromal cells. Microarray experiments detected gene probes expressed by mLN stromal cells. Transmission electron microscopy enabled us to identify lipid droplets in different stromal cells, and in macrophages. Sizes, numbers and intercellular distances increased after 10 weeks of a high-fat diet. Thus, we propose that changes in the microarchitecture and increased accumulation of lipid droplets in stromal cells and macrophages have an influence on the immunological function of the mLN.
 
Overall design Different stromal cell populations (FRC, LEC, BEC) from mesenteric lymph node (mLN) were sorted. Total RNA was prepared and subjected to single color microarray analysis (3 hybridizations in total).
 
Contributor(s) Streich K, Dittrich-Breiholz O, Buettner M
Citation(s) 32405435
https://doi.org/10.1016/j.jare.2020.04.020
Submission date Oct 08, 2019
Last update date May 18, 2020
Contact name Oliver Dittrich-Breiholz
E-mail(s) dittrich.oliver@mh-hannover.de
Organization name Medical School Hannover
Department Research Core Unit Genomics
Street address Carl-Neuberg-Str. 1
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (3)
GSM4114014 mLN FRC
GSM4114015 mLN LEC
GSM4114016 mLN BEC
This SubSeries is part of SuperSeries:
GSE138595 Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of the mesenteric lymph node
Relations
BioProject PRJNA576476

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138594_RAW.tar 27.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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