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Series GSE139222 Query DataSets for GSE139222
Status Public on Oct 22, 2019
Title Deregulated Adhesion Program in Palatal Keratinocytes of Orofacial Cleft Patients
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Orofacial clefts (OFCs) are the most frequent craniofacial birth defects. An orofacial cleft (OFC) occurs as a result of deviations in palatogenesis. Cell proliferation, differentiation, adhesion, migration and apoptosis are crucial in palatogenesis. We hypothesized that deregulation of these processes in oral keratinocytes contributes to OFC. We performed microarray expression analysis on palatal keratinocytes from OFC and non-OFC individuals. Principal component analysis showed a clear difference in gene expression with 24 and 17% for the first and second component respectively. In OFC cells, 228 genes were differentially expressed (p<0.001). Gene ontology analysis showed enrichment of genes involved in β1 integrin-mediated adhesion and migration, as well as in P-cadherin expression. A scratch assay demonstrated reduced migration of OFC keratinocytes (343.6 ± 29.62 μm) vs. non-OFC keratinocytes (503.4 ± 41.81 μm, p<0.05). Our results indicate that adhesion and migration are deregulated in OFC keratinocytes, which might contribute to OFC pathogenesis.
Overall design We performed microarray expression analysis and QPCR on palatal keratinocytes from OFC and non-OFC individuals. In addition a functional scratch assay was performed.
Microarray expression study, cultured keratinocytes, QPCR, scratch assay
Contributor(s) Mammadova A, Carels C, Zhou J, Gilissen C, Helmich M, Bian Z, Zhou H, Von den Hoff J
Citation(s) 31652793
Submission date Oct 21, 2019
Last update date Jun 14, 2022
Contact name Jo Huiqing Zhou
Organization name Radboud University
Street address Geert Grooteplein 26/28
City Nijmegen
ZIP/Postal code 6525GA
Country Netherlands
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (10)
GSM4134295 Oral keratinocytes, C4
GSM4134296 Oral keratinocytes, P5
GSM4134297 Oral keratinocytes, C3
BioProject PRJNA578774

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Supplementary file Size Download File type/resource
GSE139222_RAW.tar 47.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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