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Series GSE139399 Query DataSets for GSE139399
Status Public on Dec 15, 2019
Title Ribosome profiling study of Miwi-null testes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary piRNAs are a novel class of small noncoding RNAs that were recently identified in animal germ cells. This class of small noncoding RNAs is specifically associated with an evolutionarily conserved PIWI family proteins, which belong to the germline-specific members of the Argonaute protein family and are indispensable to germline development in animals. The PIWI/piRNA pathway has been deemed as an innate immune system that prevents mobile genetic elements from destabilizing DNA and that protects genome integrity in animal germ cells. By ribosome profiling using Miwi-null testes, we identified a group of ~600 mRNAs as likely direct piRNA targets for translational regulation in mouse spermatids. These results suggest that the mouse PIWI (MIWI)/piRNA is responsible for activating translation of a subset of spermiogenic mRNAs to coordinate with morphological transformation into elongated spermatids.
Overall design 8 biological samples are included in the study. These include RNA-seq and ribosome profiling in 24 dpp wild-type and Miwi-null testes.
Web link
Contributor(s) Dai P, Wang X, Gou L, Li Z, Wen Z, Chen Z
Citation(s) 31835033
Submission date Oct 25, 2019
Last update date Dec 16, 2019
Contact name Zonggui Chen
Organization name Peking University
Department Academy for Advanced Interdisciplinary Studies
Lab Fuchou Tang
Street address No.5 Yiheyuan Road Haidian District
City Beijing
State/province Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (8)
GSM4140495 WT input rep1
GSM4140496 WT input rep2
GSM4140497 Miwi-null input rep1
BioProject PRJNA579549
SRA SRP227006

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Supplementary file Size Download File type/resource
GSE139399_different_translation_efficiency_merged.tsv.gz 5.6 Mb (ftp)(http) TSV
GSE139399_different_translation_efficiency_rep1.tsv.gz 5.4 Mb (ftp)(http) TSV
GSE139399_different_translation_efficiency_rep2.tsv.gz 5.4 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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