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Status |
Public on Apr 14, 2021 |
Title |
Comparison of gene expression in symbiotic and non-symbiotic cells of Exaiptasia pallida |
Organism |
Exaiptasia diaphana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Emergence of the symbiotic lifestyle fostered the immense diversity of all ecosystems on Earth, but symbiosis plays a particularly remarkable role in marine ecosystems. Photosynthetic dinoflagellate endosymbionts power reef ecosystems by transferring vital nutrients to their coral hosts. The mechanisms driving this symbiosis, specifically those which allow hosts to discriminate between beneficial symbionts and pathogens, are not well understood. Here, we uncover that host immune suppression is key for dinoflagellate endosymbionts to avoid elimination by the host using a comparative, model systems approach. Unexpectedly, we find that the clearance of non-symbiotic microalgae occurs by non-lytic expulsion (vomocytosis) and not intracellular digestion, the canonical mechanism used by professional immune cells to destroy foreign invaders. We provide evidence that suppression of TLR signalling by targeting the conserved MyD88 adapter protein has been co-opted for this endosymbiotic lifestyle, suggesting that this is an evolutionarily ancient mechanism exploited to facilitate symbiotic associations ranging from coral endosymbiosis to the microbiome of vertebrate guts.
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Overall design |
The gene expression profiles of symbiotic and aposymbiotic endodermal cells obtained 24 - 48 hours after symbiosis establishment in Aiptaisia larvae were compared. In addition to GEO accession: GSE141133
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Contributor(s) |
Voss PA, Gornik SG, Jacobovitz MR, Rupp S, Maegele I, Guse A |
Citation(s) |
33927382 |
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Submission date |
Nov 08, 2019 |
Last update date |
Jul 14, 2021 |
Contact name |
Annika Guse |
Organization name |
Heidelberg University
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Department |
Centre for Organismal Studies
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Street address |
Im Neuenheimer Feld 230
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City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
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Platforms (1) |
GPL27731 |
Illumina NextSeq 500 (Exaiptasia pallida) |
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Samples (16)
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Relations |
BioProject |
PRJNA588472 |
SRA |
SRP229372 |