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Series GSE140173 Query DataSets for GSE140173
Status Public on Apr 14, 2021
Title Comparison of gene expression in symbiotic and non-symbiotic cells of Exaiptasia pallida
Organism Exaiptasia diaphana
Experiment type Expression profiling by high throughput sequencing
Summary Emergence of the symbiotic lifestyle fostered the immense diversity of all ecosystems on Earth, but symbiosis plays a particularly remarkable role in marine ecosystems. Photosynthetic dinoflagellate endosymbionts power reef ecosystems by transferring vital nutrients to their coral hosts. The mechanisms driving this symbiosis, specifically those which allow hosts to discriminate between beneficial symbionts and pathogens, are not well understood. Here, we uncover that host immune suppression is key for dinoflagellate endosymbionts to avoid elimination by the host using a comparative, model systems approach. Unexpectedly, we find that the clearance of non-symbiotic microalgae occurs by non-lytic expulsion (vomocytosis) and not intracellular digestion, the canonical mechanism used by professional immune cells to destroy foreign invaders. We provide evidence that suppression of TLR signalling by targeting the conserved MyD88 adapter protein has been co-opted for this endosymbiotic lifestyle, suggesting that this is an evolutionarily ancient mechanism exploited to facilitate symbiotic associations ranging from coral endosymbiosis to the microbiome of vertebrate guts.
 
Overall design The gene expression profiles of symbiotic and aposymbiotic endodermal cells obtained 24 - 48 hours after symbiosis establishment in Aiptaisia larvae were compared. In addition to GEO accession: GSE141133
 
Contributor(s) Voss PA, Gornik SG, Jacobovitz MR, Rupp S, Maegele I, Guse A
Citation(s) 33927382
Submission date Nov 08, 2019
Last update date Jul 14, 2021
Contact name Annika Guse
Organization name Heidelberg University
Department Centre for Organismal Studies
Street address Im Neuenheimer Feld 230
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platforms (1)
GPL27731 Illumina NextSeq 500 (Exaiptasia pallida)
Samples (16)
GSM4155745 apoapo rep1
GSM4155746 apoapo rep2
GSM4155747 apoapo rep3
Relations
BioProject PRJNA588472
SRA SRP229372

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE140173_TMM_all_replicates.xlsx 2.9 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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