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Series GSE140294 Query DataSets for GSE140294
Status Public on Oct 02, 2020
Title An intact heterotrimeric G-Protein complex is required for the N-acylethanolamine-induced, transcriptionally-mediated chloroplast response in developing Arabidopsis seedlings
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Using RNA-seq and differential expression analysis, we examined the NAE 18:3-induced and AGB1-dependent and -independent transcriptional responses involved in transducing early signals into downstream physiological changes involved in Arabidopsis seedling growth.
Methods: Seedling mRNA profiles of 4-day-old Arabidopsis seedlings treated with DMSO or 40 µM NAE 18:3 for 1 h or 3 h were generated by deep sequencing, in triplicate, using the Illumina Next-Seq 500 system. The sequence reads that passed quality filters were analyzed using STAR followed by DESeq2. qRT–PCR validation was performed using SYBR Green assays.
Results: We show that the activity of N-linolenoylethanolamine (NAE 18:3) requires an intact G-protein complex; specifically, genetic ablation of the Gβ dimer or loss of the full set of atypical G⍺ subunits strongly attenuates an NAE 18:3 de-greening of cotyledons in Arabidopsis seedlings. This effect involves, at least in part, transcriptional regulation of chlorophyll synthesis gene expression. In addition, there is feedforward transcriptional control of G-protein signaling components and G-protein interactors.
Conclusions: These results are consistent with NAE 18:3 being a lipid signaling molecule in plants with a requirement for G-proteins to mediate signal transduction, a situation similar, but not identical, to the action of NAE endocannabinoids in animal systems.
Overall design Seedling mRNA profiles of 4-day-old Arabidopsis Col-0 and agb1 seedlings treated with DMSO or 40 µM NAE 18:3 for 1 h or 3 h were generated by deep sequencing, in triplicate, using the Illumina Next-Seq 500 system.
Contributor(s) Yan C, Cannon AE, Keereetaweep J, Khan BR, Jones AM, Blancaflor EB, Azad RK, Chapman KD
Citation(s) 32665332
Submission date Nov 12, 2019
Last update date Oct 02, 2020
Contact name Ashley Elisabeth Cannon
Organization name The University of North Texas
Department Biological Sciences
Lab Chapman
Street address 1155 Union Circle #305220
City Denton
State/province TX
ZIP/Postal code 76203-5017
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (24)
GSM4159013 DMSO 1 h Col01
GSM4159014 DMSO 1 h Col02
GSM4159015 DMSO 1 h Col03
BioProject PRJNA589109
SRA SRP229635

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Supplementary file Size Download File type/resource
GSE140294_1h_counttable.txt.gz 608.4 Kb (ftp)(http) TXT
GSE140294_1h_normalized_countstable.csv.gz 2.1 Mb (ftp)(http) CSV
GSE140294_3h_counttable.txt.gz 582.2 Kb (ftp)(http) TXT
GSE140294_3h_normalized_countstable.csv.gz 2.0 Mb (ftp)(http) CSV
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Processed data are available on Series record

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