GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE140317 Query DataSets for GSE140317
Status Public on Nov 14, 2019
Title 2-Methylcitrate cycle: a well-regulated controller in Bacillus sporulation
Organism Bacillus thuringiensis BMB171
Experiment type Expression profiling by high throughput sequencing
Summary To understand which gene(s) are affected by 2-methylcitrate to lead to the delayed sporulation in ∆prpD, we extracted the total RNA from BMB171 and ∆prpD at 12 h for whole genome transcription analysis by RNA sequencing (RNA-Seq). Our RNA-seq data showed that transcriptions of almost all of the SigF regulon genes, as concluded from the B. subtilis regulon, were significantly down-regulated in ∆prpD compared with the wild type BMB171. However, almost all the regulon genes of SigH, a sigma factor that functions upstream of SigF, were significantly up-regulated in ∆prpD. Those genes included spo0A, the early-expressed operon spoIIA and sigF itself. These results indicated that dysregulation of genes occurred on various levels. In particular, though, post-transcriptional inhibition of sigF functionality might subsequently lead to down-regulation of SigF regulon genes in the ∆prpD mutant.
Overall design BMB171 and ΔprpD strains were cultured in GYS medium for 12 h, RNA samples were isolated and sequenced, each with two repetitions.
Contributor(s) Zheng C, He J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 13, 2019
Last update date Nov 20, 2019
Contact name Xinfeng Li
Organization name Huazhong Agricultural University
Street address Shi zi shan street
City Wuhan
ZIP/Postal code 430070
Country China
Platforms (1)
GPL27747 Illumina HiSeq 2000 (Bacillus thuringiensis BMB171)
Samples (4)
GSM4159195 BMB171-1
GSM4159196 BMB171-2
GSM4159197 ΔprpD-1
BioProject PRJNA589283
SRA SRP229728

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140317_expression.xlsx 376.1 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap