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Series GSE140317 Query DataSets for GSE140317
Status Public on Nov 14, 2019
Title 2-Methylcitrate cycle: a well-regulated controller in Bacillus sporulation
Organism Bacillus thuringiensis BMB171
Experiment type Expression profiling by high throughput sequencing
Summary To understand which gene(s) are affected by 2-methylcitrate to lead to the delayed sporulation in ∆prpD, we extracted the total RNA from BMB171 and ∆prpD at 12 h for whole genome transcription analysis by RNA sequencing (RNA-Seq). Our RNA-seq data showed that transcriptions of almost all of the SigF regulon genes, as concluded from the B. subtilis regulon, were significantly down-regulated in ∆prpD compared with the wild type BMB171. However, almost all the regulon genes of SigH, a sigma factor that functions upstream of SigF, were significantly up-regulated in ∆prpD. Those genes included spo0A, the early-expressed operon spoIIA and sigF itself. These results indicated that dysregulation of genes occurred on various levels. In particular, though, post-transcriptional inhibition of sigF functionality might subsequently lead to down-regulation of SigF regulon genes in the ∆prpD mutant.
 
Overall design BMB171 and ΔprpD strains were cultured in GYS medium for 12 h, RNA samples were isolated and sequenced, each with two repetitions.
 
Contributor(s) Zheng C, He J
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Submission date Nov 13, 2019
Last update date Nov 20, 2019
Contact name Xinfeng Li
E-mail(s) xinfengl@163.com
Organization name Huazhong Agricultural University
Street address Shi zi shan street
City Wuhan
ZIP/Postal code 430070
Country China
 
Platforms (1)
GPL27747 Illumina HiSeq 2000 (Bacillus thuringiensis BMB171)
Samples (4)
GSM4159195 BMB171-1
GSM4159196 BMB171-2
GSM4159197 ΔprpD-1
Relations
BioProject PRJNA589283
SRA SRP229728

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Supplementary file Size Download File type/resource
GSE140317_expression.xlsx 376.1 Kb (ftp)(http) XLSX
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Processed data are available on Series record

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