|
| Status |
Public on Jan 20, 2010 |
| Title |
E. coli BW25113 ompA mutant vs wild type biofilm and E. coli BW25113 ompA/pCA24N_ompA vs ompA/pCA24N suspension cells |
| Platform organisms |
Escherichia coli; Escherichia coli K-12 |
| Sample organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
|
| Summary |
This Series involves two studies: 1) The gene expression of E. coli K-12 BW25113 ompA mutant strain vs. wild type strain glasswool biofilm cells and E. coli K-12 BW25113 ompA mutant vs. wild type polystyrene biofilm cells. 2) The gene expression of E. coli BW25113 ompA/pCA24N_ompA vs. ompA/pCA24N suspension cells.
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| |
|
| Overall design |
Strains: E. coli K-12 BW25113 wild type, ompA mutant
Medium: LB
Cell type: Biofilm cells grown on glasswool and polystyrene surfaces
Time: 15 h
Temperature: 37C
Strains: BW25113 ompA/pCA24N_ompA and ompA/pCA24N
Medium: LB
Time: 7 h
Temperature: 37C
Cell type: suspension cells, induced by 0.1 mM IPTG
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| |
|
| Contributor(s) |
Ma Q, Wood TK |
| Citation(s) |
19601955 |
| |
| Submission date |
Dec 19, 2008 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Thomas K. Wood |
| E-mail(s) |
twood@engr.psu.edu
|
| Phone |
814-863-4811
|
| Organization name |
Pennsylvania State University
|
| Department |
Chemical Engineering & Biochemistry and Molecular Biology
|
| Lab |
Dr. Wood's Lab
|
| Street address |
Room 161 Fenske Laboratory, University Park
|
| City |
State College |
| State/province |
PA |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL199 |
[Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
|
| Samples (8)
|
| GSM352906 |
BW25113 wild type polystyrene biofilm cells |
| GSM352907 |
BW25113 ompA glasswool biofilm cells |
| GSM352909 |
BW25113 wild type glasswool biofilm cells |
| GSM414725 |
BW25113 w/t LB 15 h 37C polystyrene biofilm cell (repeat) |
| GSM414728 |
BW25113 ompA mutant LB 15 h 37C polystyrene biofilm cell (repeat) |
|
| Relations |
| BioProject |
PRJNA112433 |
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