GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE140778 Query DataSets for GSE140778
Status Public on Dec 24, 2021
Title Cellular trajectory analysis links tissue maturation to cellular specialization in the plant meristem
Organisms Arabidopsis thaliana; blank sample
Experiment type Expression profiling by high throughput sequencing
Summary The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we combine long-term imaging, single-cell transcriptomics, genetic analysis, and network modeling to reconstruct the differentiation trajectory of the 20 protophloem cells in the Arabidopsis root. We found that lineage bifurcation and cellular specification are mediated near the stem cell niche by PEAR regulators. However, many PEAR downstream effects are repressed by a broad gradient of PLETHORA transcription factors, which directly inhibit PEARs’ own direct target APL until dissipating seven cells from the stem cell niche. Modeling and molecular epistasis suggest a so-called “seesaw” cascade of sharp transitions in which transcription factors regulating consecutive steps mutually inhibit each other’s targets, ending in programmed enucleation. This analysis provides a mechanistic understanding of how morphogen-like maturation gradients interface with cell-type specific transcriptional regulators to stage cellular differentiation
Overall design Isolation and RNA seq of developing phloem cells in the root meristem of Arabidopsis thaliana
Contributor(s) Blob B, Heo J, Lau WW, Roszak P, Hamey F, Birnbaum KD, Cirrone J, Ahnert S, Göttgens B, Helariutta Y
Citation(s) 34941412
Submission date Nov 21, 2019
Last update date Mar 26, 2022
Contact name Bernhard Blob
Organization name Sainsbury Laboratory, Cambridge University
Street address 47 Bateman Street
City Cambridge
ZIP/Postal code CB21LR
Country United Kingdom
Platforms (2)
GPL21785 Illumina HiSeq 4000 (Arabidopsis thaliana)
GPL27787 Illumina HiSeq 4000 (blank sample)
Samples (1375)
GSM4183537 SLX_12100_i701_i502
GSM4183538 SLX_12100_i701_i503
GSM4183539 SLX_12100_i701_i504
BioProject PRJNA590939
SRA SRP231150

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140778_20190115_data.txt.gz 37.6 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap