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Series GSE140929 Query DataSets for GSE140929
Status Public on Dec 03, 2020
Title Colibactin causes genomic instability and promotes Wnt independence in primary colon epithelial cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Colorectal cancer is driven by a sequential cascade of mutations known as the adenoma-carcinoma sequence. Recent studies have revealed that specific bacterial species present in the colonic microbiota can induce mutations and contribute to this malignancy. Specifically, genotoxic colibactin-producing pks+ Escherichia coli strains can induce DNA double strand breaks (DSBs) and promote tumor development in mouse models of colorectal cancer. Here, we investigated the transformation potential of colibactin by using organoids and polarized monolayers derived from primary murine colon epithelial cells and reveal striking phenotypic changes upon short-term infection. This study demonstrates the direct pro-oncogenic potential of pks+ E. coli, as such transformations in vivo could facilitate colitis-associated colorectal carcinogenesis.
 
Overall design RNAseq of mouse colorectal epithelium (Wnt independent organoids derived from pks+ infected cells and uninfected control organids)
 
Contributor(s) Iftekhar A, Sigal M, Meyer TF, Boccellato F, Berger H
Citation(s) 33579932
Submission date Nov 25, 2019
Last update date Mar 02, 2021
Contact name Thomas F Meyer
E-mail(s) tfm@mpiib-berlin.mpg.de
Phone 03028 460 404
Organization name Max Planck Institute for Infection Biology
Department Molecular Biology
Street address Chariteplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (10)
GSM4190536 2534_A: Mouse 1 WI clone 1
GSM4190537 2534_B: Mouse 1 WI clone 2
GSM4190538 2534_C: Mouse 1 WI clone 3
Relations
BioProject PRJNA591553
SRA SRP233173

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE140929_RAW.tar 6.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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