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Series GSE140946 Query DataSets for GSE140946
Status Public on Mar 05, 2020
Title Cell type composition and circuit organization of neocortical radial clones
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Excitatory neurons arising from a common progenitor establish radially-oriented clonal units in the neocortex which have been proposed to serve as elementary information processing modules. To characterize the cell types and circuit diagram within these clonal units, we performed single-cell RNA-sequencing and multi-cell patch clamp recordings of neurons derived from Nestin-positive progenitors. We found that radial clones do not appear to be fate-restricted, but instead individual clones are composed of a random sampling of the transcriptomic cell types present in a particular cortical area. The effect of lineage on synaptic connectivity depends on the type of connection tested: pairs of clonally related neurons were more likely to be connected vertically, across cortical layers, but not laterally within the same layer, compared to unrelated pairs. We propose that integration of vertical input from related neurons with lateral input from unrelated neurons may represent a developmentally programmed motif for assembling neocortical circuits.
 
Overall design Electrophysiological measurements were performed on clonal neurons from a common progenitor spanning multiple brain layers and sampled across a multitude of regions in the mouse brain. mRNA was extracted from the same cells and made into sequence ready libraries using the Patch-seq/ Smart-Seq2 library preparation method
 
Contributor(s) Hartmanis L, Sandberg R
Citation(s) 32134385
Submission date Nov 25, 2019
Last update date Mar 12, 2020
Contact name Leonard Hartmanis
E-mail(s) leonard.hartmanis@ki.se
Organization name Karolinska Institutet
Department Cell & Molecular Biology
Lab Sandberg Lab
Street address Solnav├Ągen 9
City Solna
ZIP/Postal code 17165
Country Sweden
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (206)
GSM4190968 170406_p1_a2
GSM4190969 170406_p1_a1
GSM4190970 170406_p1_a3
Relations
BioProject PRJNA591575
SRA SRP233186

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Supplementary file Size Download File type/resource
GSE140946_count_matrix.txt.gz 2.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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