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Status |
Public on Dec 14, 2020 |
Title |
HMGA2 links promoter-specific H2A.X deposition and active DNA demethylation [ChIP-Seq] |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
During transcriptional activation, chromatin undergoes structural changes with the help of various proteins such as chromatin remodeling factors and histone modifiers in order to make the DNA accessible for the transcription factors and RNA polymerase. We combined ChIP-, MNase- and RNA-sequencing analyzing the Hmga2 knock-out mice and found that HMGA2 is required for promoter specific deposition of the histone variant H2A.X. Consequently, the promoter specific H2A.X deposition resulted in an accumulation of active RNA Polymerase II at these promoters. Stimulation with TGFB1 resulted in increased accumulation of pH2A.X and expression of those “primed” target genes, whereas cells expressing no Hmga2 where not able to respond.
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Overall design |
ChIP-Seq Data from chromatin extracts after ultracentrifugation from Hmga2+/+ and Hmga2-/- mouse embryonic fibroblasts
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Contributor(s) |
Barreto G, Dobersch S, Singh I |
Citation(s) |
33594057 |
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Submission date |
Dec 02, 2019 |
Last update date |
Mar 15, 2021 |
Contact name |
Guillermo Barreto |
E-mail(s) |
guillermo.barreto@univ-lorraine.fr
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Organization name |
CNRS Director of Research
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Department |
Campus Biologie-Santé. Faculté de Médecine
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Lab |
Laboratoire IMoPA. UMR 7365 CNRS
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Street address |
9, Avenue de la Forêt de Haye ‐ BP 20199
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City |
Nancy Cedex |
ZIP/Postal code |
54505 |
Country |
France |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (10)
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This SubSeries is part of SuperSeries: |
GSE141272 |
HMGA2 links promoter-specific H2A.X deposition and active DNA demethylation |
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Relations |
BioProject |
PRJNA592988 |
SRA |
SRP234418 |