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Series GSE141271 Query DataSets for GSE141271
Status Public on Dec 14, 2020
Title HMGA2 links promoter-specific H2A.X deposition and active DNA demethylation [TGFB1-ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary During transcriptional activation, chromatin undergoes structural changes with the help of various proteins such as chromatin remodeling factors and histone modifiers in order to make the DNA accessible for the transcription factors and RNA polymerase. We combined ChIP-, MNase- and RNA-sequencing analyzing the Hmga2 knock-out mice and found that HMGA2 is required for promoter specific deposition of the histone variant H2A.X. Consequently, the promoter specific H2A.X deposition resulted in an accumulation of active RNA Polymerase II at these promoters. Stimulation with TGFB1 resulted in increased accumulation of pH2A.X and expression of those “primed” target genes, whereas cells expressing no Hmga2 where not able to respond.
 
Overall design Examination of pH2A.X levels in Hmga2+/+ and Hmga2-/- mouse embryonic fibroblasts treated with TGFB1. Further, the global enrichment of FACT in Hmga2+/+ and Hmga2-/- MEFs was assayed.
 
Contributor(s) Barreto G, Dobersch S, Günther S
Citation(s) 33594057
Submission date Dec 02, 2019
Last update date Mar 15, 2021
Contact name Guillermo Barreto
E-mail(s) guillermo.barreto@univ-lorraine.fr
Organization name CNRS Director of Research
Department Campus Biologie-Santé. Faculté de Médecine
Lab Laboratoire IMoPA. UMR 7365 CNRS
Street address 9, Avenue de la Forêt de Haye ‐ BP 20199
City Nancy Cedex
ZIP/Postal code 54505
Country France
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM4200195 Hmga2+/+ Ctrl pH2AX input
GSM4200196 Hmga2+/+ TGFB1 pH2AX input
GSM4200197 Hmga2-/- Ctrl pH2AX input
This SubSeries is part of SuperSeries:
GSE141272 HMGA2 links promoter-specific H2A.X deposition and active DNA demethylation
Relations
BioProject PRJNA592985
SRA SRP234422

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Supplementary file Size Download File type/resource
GSE141271_RAW.tar 1.2 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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