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Series GSE141703 Query DataSets for GSE141703
Status Public on Dec 15, 2020
Title LFY is pioneer transcription factor [RNA-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary This study identified LEAFY (LFY) as a pioneer transcription factor. We made use of 35S:LFY-GR, an inducible version of the LFY protein fused to the rat glucocorticoid hormone binding domain. In root explants, steroid activated LFY-GR triggers synchronous and abundant flower induction (PMID: 15225291). We combined LFY ChIP-seq, MNase-seq before and after LFY binding and time-course RNA-seq in 35S:LFY-GR root explants to characterize the role of LFY as a pioneer transcription factor.
 
Overall design Identification of 35S:LFY-GR induced genes using RNA-seq in the Ler ecotype of Arabidopsis thaliana. Root explants were treated with mock or dexamethasone solution for 1hr, 6hr or 24hr. Two biological replicates were sequenced per condition.
Web link https://doi.org/10.1101/2020.03.16.994418
 
Contributor(s) Jin R, Klasfeld S, Wagner D
Citation(s) 33504790
Submission date Dec 09, 2019
Last update date Oct 05, 2021
Contact name Doris Wagner
E-mail(s) wagnerdo@sas.upenn.edu
Phone 2158980483
Organization name University of Pennsylvania
Department Department of Biology
Lab Wagner Lab
Street address 103G Carolyn Lynch Laboratory
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (12)
GSM4211294 1hrMockA RNA-Seq
GSM4211295 1hrMockB RNA-Seq
GSM4211296 1hrDexA RNA-Seq
This SubSeries is part of SuperSeries:
GSE141706 LFY is pioneer transcription factor
Relations
BioProject PRJNA594413
SRA SRP235374

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE141703_RAW.tar 211.8 Mb (http)(custom) TAR (of BIGWIG, TXT)
GSE141703_RNA1HrDex.bigwig 25.0 Mb (ftp)(http) BIGWIG
GSE141703_RNA1HrMock.bigwig 25.7 Mb (ftp)(http) BIGWIG
GSE141703_RNA24HrDex.bigwig 20.9 Mb (ftp)(http) BIGWIG
GSE141703_RNA24HrMock.bigwig 15.1 Mb (ftp)(http) BIGWIG
GSE141703_RNA6HrDex.bigwig 22.5 Mb (ftp)(http) BIGWIG
GSE141703_RNA6HrMock.bigwig 26.3 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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