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| Status |
Public on Oct 27, 2020 |
| Title |
LFY is pioneer transcription factor [ChIP-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
This study identified LEAFY (LFY) as a pioneer transcription factor. We made use of 35S:LFY-GR, an inducible version of the LFY protein fused to the rat glucocorticoid hormone binding domain. In root explants, steroid activated LFY-GR triggers synchronous and abundant flower induction (PMID: 15225291). We combined LFY ChIP-seq, MNase-seq before and after LFY binding and time-course RNA-seq in 35S:LFY-GR root explants to characterize the role of LFY as a pioneer transcription factor.
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| Overall design |
Identification of genomic regions bound by LFY in root explants identified using 35SLFY-GR in L.er ecotype of Arabidopsis thaliana. ChIP seq was performed using anti LFY antibodies 24 hours after dexamethasone or mock treatment. Three biological replicates were sequenced for each condition, as were three inputs.
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| Web link |
https://doi.org/10.1101/2020.03.16.994418
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| |
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| Contributor(s) |
Jin R, Klasfeld S, Wagner D |
| Citation(s) |
33504790 |
| |
| Submission date |
Dec 09, 2019 |
| Last update date |
Oct 05, 2021 |
| Contact name |
Doris Wagner |
| E-mail(s) |
wagnerdo@sas.upenn.edu
|
| Phone |
2158980483
|
| Organization name |
University of Pennsylvania
|
| Department |
Department of Biology
|
| Lab |
Wagner Lab
|
| Street address |
103G Carolyn Lynch Laboratory
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE141706 |
LFY is pioneer transcription factor |
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| Relations |
| BioProject |
PRJNA594412 |
| SRA |
SRP235377 |
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