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Status |
Public on Jan 30, 2020 |
Title |
Binding and regulation of transcription by yeast Ste12 variants to drive mating and invasion phenotypes |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by high throughput sequencing Other
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Summary |
Here, we examine how six single amino acid variants in the DNA-binding domain of Ste12 – a yeast transcription factor regulating mating and invasion – alter Ste12 genome binding, motif recognition and gene expression to yield markedly different phenotypes. Using a combination of the calling card method, RNA sequencing , we find that variants with dissimilar binding and expression profiles can converge onto similar cellular behaviors.
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Overall design |
We overexpress six variant of transcription factor Ste12 in yeast S.cervesiae and detected its genome-wide binding pattern and RNA expression pattern
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Contributor(s) |
Zhou W, Dorrity MW, Bubb KL, Queitsch C, Fields S |
Citation(s) |
31810988 |
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Submission date |
Dec 09, 2019 |
Last update date |
Apr 30, 2020 |
Contact name |
wei zhou |
E-mail(s) |
wei1025@uw.edu
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Phone |
2075460500
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Organization name |
University of Washington
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Department |
genome sciences
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Lab |
Stanley Fields
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Street address |
4747 30th ave NE
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City |
seattle |
State/province |
wa |
ZIP/Postal code |
98105 |
Country |
USA |
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Platforms (1) |
GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
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Samples (18)
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Relations |
BioProject |
PRJNA595929 |
SRA |
SRP237782 |