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| Status |
Public on Mar 11, 2021 |
| Title |
Immunotherapy against amyloid beta is modulated by meningeal lymphatic function II |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A growing body of evidence points to passive immunotherapy as a promising therapeutic strategy against AD. Herein, we show that meningeal lymphatic vasculature becomes dysfunctional in AD transgenic mice and that manipulating meningeal lymphatic drainage affects the outcome of anti-Aβ immunotherapy.
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| Overall design |
At the age of 4 months, male 5xFAD mice were subjected to an intracisterna magna injection of Visudyne (5 uL) alone (control), or injection of Visudyne (5 uL) followed by 5 steps of transcranial photoconversion using a 689-nm-wavelength non-thermal red light (to induce meningeal lymphatic vessel ablation). Injections were repeated 3 weeks later and mice were euthanized 3 weeks after the last injection. Microglia from the brain cortex were sorted by FACS based on the following phenotype: Singlets SYTOX-Ly6G-CD45+CD11b+. Each individual sample results from brain cortical cells pooled from 3 mice.
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| Contributor(s) |
Da Mesquita S, Papadopoulos Z, Dykstra T, Wall M, Farias FG, Brase L, Jiang H, Kodira CD, Alves de Lima K, Herz J, Louveau A, Goldman DH, Salvador AF, Onengut-Gumuscu S, Farber E, Dabhi N, Kennedy T, Milam M, Baker W, Smirnov I, Rich SS, Holtzman DM, Cruchaga C, Harari O, Kipnis J |
| Citation(s) |
33911285, 37085492 |
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| Submission date |
Dec 11, 2019 |
| Last update date |
May 12, 2023 |
| Contact name |
Jonathan Kipnis |
| Organization name |
Washington University in Saint Louis
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| Department |
Pathology and Immunology
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| Lab |
Kipnis Lab
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| Street address |
4515 McKinley Ave
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| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE141917 |
Immunotherapy against amyloid beta is modulated by meningeal lymphatic function |
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| Relations |
| BioProject |
PRJNA594991 |
| SRA |
SRP237263 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE141872_RAW.tar |
4.7 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE141872_all_counts.csv.gz |
3.1 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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