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Series GSE141872 Query DataSets for GSE141872
Status Public on Mar 11, 2021
Title Immunotherapy against amyloid beta is modulated by meningeal lymphatic function II
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A growing body of evidence points to passive immunotherapy as a promising therapeutic strategy against AD. Herein, we show that meningeal lymphatic vasculature becomes dysfunctional in AD transgenic mice and that manipulating meningeal lymphatic drainage affects the outcome of anti-Aβ immunotherapy.
Overall design At the age of 4 months, male 5xFAD mice were subjected to an intracisterna magna injection of Visudyne (5 uL) alone (control), or injection of Visudyne (5 uL) followed by 5 steps of transcranial photoconversion using a 689-nm-wavelength non-thermal red light (to induce meningeal lymphatic vessel ablation). Injections were repeated 3 weeks later and mice were euthanized 3 weeks after the last injection. Microglia from the brain cortex were sorted by FACS based on the following phenotype: Singlets SYTOX-Ly6G-CD45+CD11b+. Each individual sample results from brain cortical cells pooled from 3 mice.
Contributor(s) Da Mesquita S, Papadopoulos Z, Dykstra T, Wall M, Farias FG, Brase L, Jiang H, Kodira CD, Alves de Lima K, Herz J, Louveau A, Goldman DH, Salvador AF, Onengut-Gumuscu S, Farber E, Dabhi N, Kennedy T, Milam M, Baker W, Smirnov I, Rich SS, Holtzman DM, Cruchaga C, Harari O, Kipnis J
Citation(s) 33911285, 37085492
Submission date Dec 11, 2019
Last update date May 12, 2023
Contact name Jonathan Kipnis
Organization name Washington University in Saint Louis
Department Pathology and Immunology
Lab Kipnis Lab
Street address 4515 McKinley Ave
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM4215248 Visudyne
GSM4215249 Visudyne/photoconversion
This SubSeries is part of SuperSeries:
GSE141917 Immunotherapy against amyloid beta is modulated by meningeal lymphatic function
BioProject PRJNA594991
SRA SRP237263

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Supplementary file Size Download File type/resource
GSE141872_RAW.tar 4.7 Mb (http)(custom) TAR (of MTX, TSV)
GSE141872_all_counts.csv.gz 3.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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