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Series GSE141937 Query DataSets for GSE141937
Status Public on Dec 07, 2020
Title Effect of dietary n-3 PUFA supplementation and post-insemination plane of nutrition on global gene expression patterns in bovine uterine endometrial tissue
Organism Bos taurus
Experiment type Expression profiling by high throughput sequencing
Summary The objectives of this study were to examine the effect of (i) dietary supplementation with n-3 PUFA and (ii) post-insemination plane of nutrition on the global transcriptome of uterine endometrial tissue, with particular focus on key genes and pathways that may affect pregnancy outcome. A total of 60 estrous-synchronised continental crossbred heifers were offered a concentrate-based diet supplemented (n-3 PUFA; n=32) or not (Control; n=28) with an n-3 PUFA-enriched product for 30 days prior to insemination. Following insemination, animals were allocated, within treatment, to one of two post insemination diets, either remaining on their existing high plane of nutrition (Control + High (n = 14); n-3 PUFA + High (n = 15)), or fed a low plane of nutrition diet (Control + Low (n = 14); n-3 PUFA + Low (n = 17)). Uterine endometrial tissue was collected, RNA isolated and gene expression analysis conducted using RNAseq. Comparison of transcript abundance across groups, highlighted a significant effect of diet on the endometrial transcriptome. The mTOR signalling pathway as well as pathways involved in early pregnancy/fertility was significantly enriched as a consequence of diet.
 
Overall design A total of 60 estrous-synchronised continental crossbred heifers were offered a concentrate-based diet supplemented (n-3 PUFA; n=32) or not (Control; n=28) with an n-3 PUFA-enriched product for 30 days prior to insemination. Following insemination, animals were allocated, within treatment, to one of two post insemination diets, either remaining on their existing high plane of nutrition (Control + High (n = 14); n-3 PUFA + High (n = 15)), or fed a low plane of nutrition diet (Control + Low (n = 14); n-3 PUFA + Low (n = 17)). Uterine endometrial tissue samples were collected from all heifers following 16 days of differential feeding during the post-insemination phase.
 
Contributor(s) Surlis C, Cormican P, Waters S, Lonergan P, Doyle D, Keogh K, Kenny D
Citation(s) 33247230
Submission date Dec 12, 2019
Last update date Dec 07, 2020
Contact name Kate Keogh
Organization name Teagasc Grange
Department Animal and Bioscience Department
Street address Dunsany
City Co. Meath
ZIP/Postal code 0000
Country Ireland
 
Platforms (1)
GPL15749 Illumina HiSeq 2000 (Bos taurus)
Samples (60)
GSM4216620 control 1 Low diet
GSM4216621 control 2 Low diet
GSM4216622 control 3 Low diet
Relations
BioProject PRJNA595353
SRA SRP237353

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Supplementary file Size Download File type/resource
GSE141937_merged_counts.txt.gz 1.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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