|
| Status |
Public on Dec 17, 2019 |
| Title |
Interaction of Va and Vc in somatic cell reprogramming |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Since VitaminA and VitaminC are pleiotropic vitamins, and both can promote reprogramming, we want to know the relationship between them. We use serum-free N2B27(B27 minus Va) medium to generate iPS with OKSM virus infecting OG2 MEF, and collect mRNA at day7 under 10nM and 1uM Va with or without Vc. We found tha both Va and Vc promote reprogramming, and the genes upregulated by Va and Va had overlpped more than 80%.
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| |
|
| Overall design |
To detecn the interaction of Va and Vc in MEFs during reprogramming, we collect iPS samples at day7 with Va or Vc in the medium and process with RNA-seq.
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| |
|
| Contributor(s) |
Zhang M, Zheng H |
| Citation(s) |
33000315 |
| |
| Submission date |
Dec 16, 2019 |
| Last update date |
Oct 27, 2020 |
| Contact name |
Zheng Hui |
| E-mail(s) |
zhang_mengdan@gibh.ac.cn
|
| Organization name |
GIBH
|
| Street address |
Kaiyuan Avenue 190
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (13)
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|
| Relations |
| BioProject |
PRJNA595943 |
| SRA |
SRP237787 |
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