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Status |
Public on Dec 17, 2019 |
Title |
Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri |
Organism |
Panonychus citri |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The citrus red mite, Panonychus citri, is a major pest on citrus all around the world. Mitochondrial Electron Transport Inhibitors of complex I (METI-I) acaricides such as fenpyroximate have been used extensively to control P. citri populations, which resulted in multiple reports of METI-I resistant populations in the field. In this study, gene expression profile differences between METI-I resistant P. citri populations (Lahijan, Ramsar and Sari) and a susceptible P. citri population (Rasht) from Iran were compared using Illumina RNAseq.
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Overall design |
In total 16 RNA-seq samples (4 populations, 4 biological replicates per population) from Panonychus citri, were generated. RNA was collected from adult P. ctiri females. For each sample, Illumina mRNA-seq libraries were constructed and strand-specific, paired-end 125 bp RNA-seq Illumina reads were generated. The obtained reads were used to quantify gene expresison in each sample.
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Web link |
https://doi.org/10.1016/j.pestbp.2019.12.009
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Contributor(s) |
Snoeck S, Alavijeh ES, Khajehali J, Van Leeuwen T, Dermauw W |
Citation |
Alavijeh ES, Khajehali J, Snoeck S, Panteleri R, Ghadamyari M, Jonckheere W, Bajda S, Saalwaechter C, Geibel S, Douris V, Vontas J, Van Leeuwen T, Dermauw W. Molecular and genetic analysis of resistance to METI-I acaricides in Iranian populations of the citrus red mite Panonychus citri, Pesticide Biochemistry and Physiology, 2020.
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Submission date |
Dec 16, 2019 |
Last update date |
Mar 17, 2020 |
Contact name |
Wannes Dermauw |
E-mail(s) |
wannes.dermauw@ugent.be
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Phone |
003292646192
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Organization name |
University Ghent
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Department |
Crop Protection
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Lab |
Agrozoology
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Street address |
Coupure Links 653
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City |
Ghent |
ZIP/Postal code |
9000 |
Country |
Belgium |
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Platforms (1) |
GPL27920 |
Illumina HiSeq 2500 (Panonychus citri) |
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Samples (16)
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Relations |
BioProject |
PRJNA595971 |
SRA |
SRP237799 |
Supplementary file |
Size |
Download |
File type/resource |
GSE142139_abundance_all.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
GSE142139_de_novo_transcriptome_P_citri_used_for_Kallisto_pseudoalignment.fa.gz |
8.6 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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