GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE142259 Query DataSets for GSE142259
Status Public on Dec 24, 2021
Title Cellular trajectory analysis links tissue maturation to cellular specialization in the plant meristem [protophloem sieve element cells]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we combine long-term imaging, single-cell transcriptomics, genetic analysis, and network modeling to reconstruct the differentiation trajectory of the 20 protophloem cells in the Arabidopsis root. We found that lineage bifurcation and cellular specification are mediated near the stem cell niche by PEAR regulators. However, many PEAR downstream effects are repressed by a broad gradient of PLETHORA transcription factors, which directly inhibit PEARs’ own direct target APL until dissipating seven cells from the stem cell niche. Modeling and molecular epistasis suggest a so-called “seesaw” cascade of sharp transitions in which transcription factors regulating consecutive steps mutually inhibit each other’s targets, ending in programmed enucleation. This analysis provides a mechanistic understanding of how morphogen-like maturation gradients interface with cell-type specific transcriptional regulators to stage cellular differentiation
Overall design RNA seq profiling of bulk-isolated (FACS) protophloem sieve element cells
Contributor(s) Blob B, Heo J, Angels de Luis Balaguer M, Sozzani R, Helariutta Y
Citation(s) 34941412
Submission date Dec 18, 2019
Last update date Mar 26, 2022
Contact name Bernhard Blob
Organization name Sainsbury Laboratory, Cambridge University
Street address 47 Bateman Street
City Cambridge
ZIP/Postal code CB21LR
Country United Kingdom
Platforms (1)
GPL17639 Illumina HiSeq 2500 (Arabidopsis thaliana)
Samples (21)
GSM4223959 CD_1
GSM4223960 CD_2
GSM4223961 CD_3
BioProject PRJNA596429
SRA SRP238084

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142259_RAW.tar 2.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap