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| Status |
Public on Sep 18, 2020 |
| Title |
Modulation of immune system mediated by ibrutinib in chronic lymphocytic leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Our project is based on the hypothesis that ibrutinib could interfere with chronic lymphocytic leukemia (CLL) microenvironment, modulating the immune response. The aim of the project is to understand if and how ibrutinib modifies the tumor microenvironment accessory cells in CLL, specifically nurse like cells (NLC).
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| Overall design |
Large-scale gene expression profiling (GEP) was performed on total RNA extracted from NLC treated or not (control sample) over-night with 0.5µM Ibrutinib (RNeasy Mini kit Plus, QIAGEN, Valencia, CA, USA) from 10 individual CLL patients by hybridization on 4X44K Whole Human Genome Microarray (Agilent Technologies, Palo alto, CA). Fluorescence data were analysed with Feature Extraction Software v.10.5 (Agilent Technologies) an QC Chart tool v.1.3.
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| |
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| Contributor(s) |
Maffei R, Marasca R |
| Citation(s) |
32983178 |
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https://doi.org/10.3389/fimmu.2020.02158
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| |
| Submission date |
Dec 18, 2019 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Rossana Maffei |
| E-mail(s) |
rossana.maffei@unimore.it
|
| Phone |
+39 059 4222715
|
| Organization name |
University of Modena and Reggio Emilia
|
| Department |
Dept of Hematology and Oncology
|
| Lab |
Lab of Molecular Hematology
|
| Street address |
Via del Pozzo 71
|
| City |
Modena |
| State/province |
Modena |
| ZIP/Postal code |
41100 |
| Country |
Italy |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA596472 |
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