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Series GSE142292 Query DataSets for GSE142292
Status Public on Sep 18, 2020
Title Modulation of immune system mediated by ibrutinib in chronic lymphocytic leukemia
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Our project is based on the hypothesis that ibrutinib could interfere with chronic lymphocytic leukemia (CLL) microenvironment, modulating the immune response. The aim of the project is to understand if and how ibrutinib modifies the tumor microenvironment accessory cells in CLL, specifically nurse like cells (NLC).
 
Overall design Large-scale gene expression profiling (GEP) was performed on total RNA extracted from NLC treated or not (control sample) over-night with 0.5┬ÁM Ibrutinib (RNeasy Mini kit Plus, QIAGEN, Valencia, CA, USA) from 10 individual CLL patients by hybridization on 4X44K Whole Human Genome Microarray (Agilent Technologies, Palo alto, CA). Fluorescence data were analysed with Feature Extraction Software v.10.5 (Agilent Technologies) an QC Chart tool v.1.3.
 
Contributor(s) Maffei R, Marasca R
Citation(s) 32983178
https://doi.org/10.3389/fimmu.2020.02158
Submission date Dec 18, 2019
Last update date Oct 02, 2020
Contact name Rossana Maffei
E-mail(s) rossana.maffei@unimore.it
Phone +39 059 4222715
Organization name University of Modena and Reggio Emilia
Department Dept of Hematology and Oncology
Lab Lab of Molecular Hematology
Street address Via del Pozzo 71
City Modena
State/province Modena
ZIP/Postal code 41100
Country Italy
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (20)
GSM4224667 #1 NLC CTRL
GSM4224668 #1 NLC IBRUTINIB
GSM4224669 #2 NLC CTRL
Relations
BioProject PRJNA596472

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142292_RAW.tar 50.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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