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Series GSE142453 Query DataSets for GSE142453
Status Public on Aug 25, 2021
Title Cerebral cortex and blood transcriptome changes in mouse neonates prenatally exposed to air pollution particulate matter
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Background: Prenatal exposure to air pollutants is associated with increased risk for neurodevelopmental and neurodegenerative disorders. However, few studies have identified transcriptional changes related to air pollutant exposure.

Methods: RNA sequencing was used to examine transcriptomic changes in blood and cerebral cortex of three male and three female mouse neonates prenatally exposed to traffic-related nano-sized particulate matter (nPM) compared to three male and three female mouse neonates prenatally exposed to control filter air.

Results: We identified 19 nPM-associated differentially expressed genes (nPM-DEGs) in blood and 124 nPM-DEGs in cerebral cortex. The cerebral cortex transcriptional responses to nPM suggested neuroinflammation involvement, including CREB1, BDNF, and IFN γ genes. Both blood and brain tissues showed nPM transcriptional changes related to DNA damage, oxidative stress, and immune responses. Three blood nPM-DEGs showed a canonical correlation of 0.98 with 14 nPM-DEGS in the cerebral cortex, suggesting a convergence of gene expression changes in blood and cerebral cortex. Exploratory sex-stratified analyses suggested a higher number of nPM-DEGs in female cerebral cortex than male cerebral cortex. The sex-stratified analyses identified 2 nPM-DEGs (Rgl2 and Gm37534) shared between blood and cerebral cortex in a sex-dependent manner.

Conclusions: Our findings suggest that prenatal nPM exposure induces transcriptional changes in the cerebral cortex, some of which are also observed in blood. Further research is needed to replicate nPM-induced transcriptional changes with additional biologically relevant time points for brain development.
 
Overall design Pregrant dams were exposed to nPM during gestational period. The effects were studied in neonate pups.
Web link https://doi.org/10.1186/s11689-021-09380-3
 
Contributor(s) Haghani A, Feinberg JI, Lewis KC, Ladd-Acosta C, Johnson RG, Jaffe AE, Sioutas C, Finch CE, Campbell DB, Morgan TE, Volk HE
Citation(s) 34429070
Submission date Dec 20, 2019
Last update date Aug 25, 2021
Contact name Amin Haghani
E-mail(s) ahaghani@altoslabs.com
Organization name Altos labs
Lab Hovath lab
Street address 5510 Morehouse Dr
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platforms (1)
GPL21493 Illumina HiSeq 3000 (Mus musculus)
Samples (24)
GSM4227549 V09_HJ7M2BBXX
GSM4227550 V10_HJ7M2BBXX
GSM4227551 V11_HJ7M2BBXX
Relations
BioProject PRJNA597046
SRA SRP238413

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142453_gene_counts.csv.gz 1.0 Mb (ftp)(http) CSV
GSE142453_gene_map.csv.gz 1.7 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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