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| Status |
Public on Aug 12, 2021 |
| Title |
Genome-wide binding of Bmal1 and Clock in adult mouse skeletal muscle. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The goal of this study was to define the binding sites of the core circadian factors, BMAL1 and CLOCK across the genome in adult mouse skeletal muscle. We found that mitochondrial inner-membrane genes were among the most enriched rhythmic genes via gene ontology analysis, and ChIP-Seq analysis corroborated the role of Clock and Bmal1 in targeting promoters of mitochondrial inner-membrane genes.
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| Overall design |
We collected mouse gastrocnemius muscles from 10 adult C57BL/6J male at 2hrs after lights on (ZT2). Nuclei were extracted and proteins fixed and we used antibodies to Bmal1 and Clock for Chromatin IP. There are two biological replicates for Bmal1, two biological replicates for Clock, one input sample for Bmal1, and one input sample for Clock. Libraries were prepared using NEBNext Ultra DNA library kit and the libraries were sequenced on HiSeq3000 Illumina system.
Each processed data was generated from both replicates and is linked to the corresponding rep1 sample records.
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| Contributor(s) |
Zhang X, Huo Z, Esser K, Zierath J, Gabriel B |
| Citation(s) |
34669477 |
| |
| Submission date |
Jan 08, 2020 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Karyn A Esser |
| E-mail(s) |
kaesser@ufl.edu
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| Organization name |
University of Florida
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| Department |
PHYSIOLOGY & FUNCTIONAL GENOMICS
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| Lab |
Esser's lab
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| Street address |
1345 Center Drive
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| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA600047 |
| SRA |
SRP240731 |
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