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Series GSE143441 Query DataSets for GSE143441
Status Public on Aug 08, 2021
Title N6-adenosine Methylation of Enhancer RNAs and YTHDC1 Facilitate Transcriptional Condensate Formation and 3D Chromatin Organization
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Eukaryotic chromatin is pervasively transcribed into nascent RNAs. However, a mechanistic understanding of their general roles remains lacking. Here, we characterized the landscapes of N6-adenosine methylation (i.e. m6A) on nascent RNAs using a new method that we referred to as MINT-Seq. We found widespread m6A deposition on nascent RNAs produced from regulatory elements including enhancer RNAs (eRNAs) and promoter upstream antisense RNAs (uaRNAs).
Overall design The lanscape of m6A methylation on enhancer RNAs in human cells using MINT-Seq. We also examined the roles of m6A-eRNAs and YTHDC1 in transcriptional regulation and 3D genome structure with GRO-Seq, TT-Seq, ChIP-Seq and, Hi-C.
Contributor(s) Li W, Lee J, Wang R
Citation(s) 34375583
Submission date Jan 10, 2020
Last update date Oct 01, 2021
Contact name Ruoyu Wang
Organization name UTHealth McGovern Medical School
Street address 6431 Fannin St
State/province Texas
ZIP/Postal code 77054
Country USA
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (62)
GSM4259508 MCF7 EtOH TT-Seq rep1
GSM4259509 MCF7 EtOH MINT-Seq rep1
GSM4259510 MCF7 E2 TT-Seq rep1
BioProject PRJNA600511
SRA SRP241208

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143441_RAW.tar 9.2 Gb (http)(custom) TAR (of BED, BIGWIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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