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Series GSE143959 Query DataSets for GSE143959
Status Public on Dec 07, 2020
Title Defining a critical Nanog enhancer using chromatin-focused approaches identifies RNAPII recruitment as required for expression [-5delRNASeq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Transcriptional enhancers have been defined by their ability to operate independent of distance and orientation in plasmid reporters rather than native chromatin. We employed a combination of approaches to investigate the Nanog locus in mouse embryonic stem cells (ESCs) and an associated cis regulatory element (CRE) which may operate as either a super-enhancer (SE) or alternative promoter. We establish this element is a SE within chromatin using distance- and orientation-independence as criteria, and that it regulates Nanog by recruiting and/or initiating RNA Polymerase II. Our work highlights that enhancers can regulate gene expression through a diverse set of mechanisms.
 
Overall design RNA-seq of 2 conditions, 3 replicates of each
 
Contributor(s) Agrawal P, Rao S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 20, 2020
Last update date Dec 07, 2020
Contact name Sridhar Rao
E-mail(s) sridhar.rao@bcw.edu
Phone 4149373841
Organization name BloodCenter of Wisconsin
Department Blood Research Institute
Street address 8727 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM4277330 13etoh1
GSM4277331 13etoh2
GSM4277332 13etoh3
This SubSeries is part of SuperSeries:
GSE143993 Genome editing demonstrates that the -5 kb Nanog enhancer regulates Nanog expression by modulating RNAPII initiation and/or recruitment
Relations
BioProject PRJNA602333
SRA SRP243486

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143959_5del_DESeq.gct.gz 348.2 Kb (ftp)(http) GCT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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