GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE143991 Query DataSets for GSE143991
Status Public on Dec 07, 2020
Title Defining a critical Nanog enhancer using chromatin-focused approaches identifies RNAPII recruitment as required for expression [CUT&Tag]
Organism Mus musculus
Experiment type Other
Summary Transcriptional enhancers have been defined by their ability to operate independent of distance and orientation in plasmid reporters rather than native chromatin. We employed a combination of approaches to investigate the Nanog locus in mouse embryonic stem cells (ESCs) and an associated cis regulatory element (CRE) which may operate as either a super-enhancer (SE) or alternative promoter. We establish this element is a SE within chromatin using distance- and orientation-independence as criteria, and that it regulates Nanog by recruiting and/or initiating RNA Polymerase II. Our work highlights that enhancers can regulate gene expression through a diverse set of mechanisms.
Overall design CUT&Tag of 2 conditions, 3 replicates of each
Contributor(s) Agrawal P, Rao S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 21, 2020
Last update date Dec 07, 2020
Contact name Sridhar Rao
Phone 4149373841
Organization name BloodCenter of Wisconsin
Department Blood Research Institute
Street address 8727 Watertown Plank Road
City Milwaukee
State/province WI
ZIP/Postal code 53226
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM4277799 wt_1
GSM4277800 wt_2
GSM4277806 0copy_1
This SubSeries is part of SuperSeries:
GSE143993 Genome editing demonstrates that the -5 kb Nanog enhancer regulates Nanog expression by modulating RNAPII initiation and/or recruitment
BioProject PRJNA602469
SRA SRP243681

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143991_0copy_Ser5P_cutntag.bedgraph.gz 8.6 Mb (ftp)(http) BEDGRAPH
GSE143991_wt_Ser5P_cutntag.bedgraph.gz 8.2 Mb (ftp)(http) BEDGRAPH
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap