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Series GSE144512 Query DataSets for GSE144512
Status Public on Feb 04, 2022
Title Intercellular communication via miR-143-containing microvesicles controls osteoblastic bone formation in vivo
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Osteoblasts are the only somatic cell type with bone-forming ability. While the cellular and molecular mechanisms underlying their differentiation and function have been identified, their dynamic control in vivo is unclear. By intravital multiphoton microscopy of live bone tissues of osteoblast fluorescent reporter mice, we visualized intact mature osteoblasts in vivo and found that they actively secrete and capture extracellular microvesicles. Microvesicles from mature osteoblasts contain the microRNA miR-143-3p, which inhibits osteoblast differentiation. Osteoblast-specific deletion of miR-143 increased bone formation and miR-143-deficient microvesicles induced recovery from bone defect. In sum, we report a novel mode of intercellular communication in vivo via microRNA-containing extracellular vesicles, which controls bone homeostasis.
 
Overall design We performed next-generation sequencing analysis of small RNAs in total mOB-derived large EVs without filtration.
 
Contributor(s) Uenaka M, Okuzaki D
Citation(s) 35210428
Submission date Jan 30, 2020
Last update date Feb 28, 2022
Contact name Daisuke Okuzaki
E-mail(s) dokuzaki@biken.osaka-u.ac.jp
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (1)
GSM4289829 EV-T
Relations
BioProject PRJNA603887
SRA SRP246039

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE144512_RAW.tar 10.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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