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Status |
Public on Feb 20, 2020 |
Title |
Platform effects on regeneration by pulmonary basal cells as evaluated by single-cell RNA sequencing [array] |
Organism |
Rattus norvegicus |
Experiment type |
Expression profiling by array
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Summary |
Cell-based therapies have shown promise for treating myriad chronic pulmonary diseases through direct application of epithelial progenitors or by way of engineered tissue grafts or whole organs. To elucidate environmental effects on epithelial regenerative outcomes in vitro, we isolated and cultured a population of pharmacologically expanded basal cells (peBC) from rat tracheas. At peak basal marker expression we simultaneously split peBC into four in vitro platforms – organoid, air-liquid interface (ALI), engineered trachea, and engineered lung. Following differentiation, these samples were evaluated using single-cell RNA sequencing (scRNAseq) and computational pipelines were developed to compare samples both globally and at the population level. A sample of native rat tracheal epithelium was also evaluated by scRNAseq as a control for engineered epithelium. Overall this work identifies platform-specific effects that support the use of engineered models to achieve the most physiologic differential outcomes in pulmonary epithelial regenerative applications.
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Overall design |
Comparison of three primary rat tracheal epithelial scrapings plated into BEGM or EpiX media and grown for 8 days
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Contributor(s) |
Greaney AM |
Citation missing |
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Submission date |
Feb 19, 2020 |
Last update date |
Feb 23, 2020 |
Contact name |
Allison M Greaney |
E-mail(s) |
allison.greaney@yale.edu
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Organization name |
Yale University
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Department |
Biomedical Engineering
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Street address |
10 Amistad St
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City |
New Haven |
State/province |
CT |
ZIP/Postal code |
06511 |
Country |
USA |
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Platforms (1) |
GPL23040 |
[Clariom_S_Rat] Affymetrix Clariom S Assay, Rat (Includes Pico Assay) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE145518 |
Platform effects on regeneration by pulmonary basal cells as evaluated by single-cell RNA sequencing |
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Relations |
BioProject |
PRJNA607458 |