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Series GSE145715 Query DataSets for GSE145715
Status Public on Jan 26, 2021
Title Beyond flowering time: diverse roles of an APETALA2‐like transcription factor in shoot architecture and perennial traits
Organism Arabis alpina
Experiment type Expression profiling by high throughput sequencing
Summary Polycarpic perennials maintain vegetative growth after flowering. PERPETUAL FLOWERING 1 (PEP1), the orthologue of FLOWERING LOCUS C (FLC) inArabis alpina regulates flowering and contributes to polycarpy in a vernalisation-dependent pathway. pep1 mutants do not require vernalisation to flower and have reduced return to vegetative growth as all of their axillary branches become reproductive.
To identify additional genes that regulate flowering and contribute to perennial traits we performed an enhancer screen of pep1. Using mapping-by-sequencing, we cloned a mutant (enhancer of pep1-055, eop055), performed transcriptome analysis and physiologically characterised the role it plays on perennial traits in an introgression line carrying the eop055 mutation and a functional PEP1 wild-type allele.
eop055 flowers earlier than pep1 and carries a lesion in the A. alpina orthologue of the APETALA2 (AP2)-like gene, TARGET OF EAT2 (AaTOE2). AaTOE2 is a floral repressor and acts upstream of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 5 (AaSPL5). In the wild-type background, which requires cold treatment to flower, AaTOE2 regulates the age-dependent response to vernalisation. In addition, AaTOE2 ensures the maintenance of vegetative growth by delaying axillary meristem initiation and repressing flowering of axillary buds before and during cold exposure.
We conclude that AaTOE2 is instrumental in fine tuning different developmental traits in the perennial life cycle of A. alpina.
Overall design Main shoot apices from 8-week-old plants vernalized for eight weeks (8wLD+8wV) were collected from wild-type and Aatoe2_IL plants to study the effect of AaTOE2 at the transcriptomic level in the regulation of flowering during vernalization. Also, V3 buds were harvested from 8-week-old plants vernalized for eight weeks from both genotypes to study the role of AaTOE2 in the development of axillary branches.
Web link http://
Contributor(s) Zhou Y, Gan X, Viñegra de la Torre N, Neumann U, Albani MC
Citation(s) 32745288
Submission date Feb 21, 2020
Last update date Jan 26, 2021
Contact name Maria Albani
Organization name University of Cologne
Department Botanical institute
Street address Carl-von-Linné-Weg 10
City Köln
ZIP/Postal code 50829
Country Germany
Platforms (1)
GPL26216 Illumina HiSeq 3000 (Arabis alpina)
Samples (12)
GSM4331498 WT_shoot apex_rep1
GSM4331499 WT_shoot apex_rep2
GSM4331500 WT_shoot apex_rep3
This SubSeries is part of SuperSeries:
GSE145718 APETALA2‐like transcription factor in shoot architecture and perennial traits
BioProject PRJNA608060
SRA SRP250384

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Supplementary file Size Download File type/resource
GSE145715_Raw_counts_for_DEseq.csv.gz 537.4 Kb (ftp)(http) CSV
GSE145715_Table_S3_DEGs_in_Aatoe2_IL_vs_wild-type_in_shoot_apex_and_V3_buds.xlsx 69.5 Kb (ftp)(http) XLSX
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