 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 26, 2021 |
| Title |
Beyond flowering time: diverse roles of an APETALA2‐like transcription factor in shoot architecture and perennial traits |
| Organism |
Arabis alpina |
| Experiment type |
Genome variation profiling by high throughput sequencing
|
| Summary |
Polycarpic perennials maintain vegetative growth after flowering. PERPETUAL FLOWERING 1 (PEP1), the orthologue of FLOWERING LOCUS C (FLC) inArabis alpina regulates flowering and contributes to polycarpy in a vernalisation-dependent pathway. pep1 mutants do not require vernalisation to flower and have reduced return to vegetative growth as all of their axillary branches become reproductive.
To identify additional genes that regulate flowering and contribute to perennial traits we performed an enhancer screen of pep1. Using mapping-by-sequencing, we cloned a mutant (enhancer of pep1-055, eop055), performed transcriptome analysis and physiologically characterised the role it plays on perennial traits in an introgression line carrying the eop055 mutation and a functional PEP1 wild-type allele.
eop055 flowers earlier than pep1 and carries a lesion in the A. alpina orthologue of the APETALA2 (AP2)-like gene, TARGET OF EAT2 (AaTOE2). AaTOE2 is a floral repressor and acts upstream of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 5 (AaSPL5). In the wild-type background, which requires cold treatment to flower, AaTOE2 regulates the age-dependent response to vernalisation. In addition, AaTOE2 ensures the maintenance of vegetative growth by delaying axillary meristem initiation and repressing flowering of axillary buds before and during cold exposure.
We conclude that AaTOE2 is instrumental in fine tuning different developmental traits in the perennial life cycle of A. alpina.
|
| |
|
| Overall design |
The eop mutants were self-fertilized for two generations to fix the phenotype of interest and then backcrossed to the non-mutagenized progenitor pep1-1 followed by one generation of self-fertilization to create a BC1F2 mapping population. Within this backcross population, only mutagen-induced changes segregate, and the causal and closely linked mutations will be fixed in the mutant pool by selecting plants with the mutant phenotype (James et al., 2013). As a result, lots of putative causal changes will be reduced to improve the accuracy of mapping. Researchers suggested that the backcross population needs high coverage (>25 x) for optimal mapping as the number of markers (or mutations) is relatively lower (James et al., 2013).
|
| Web link |
http:// https://doi.org/10.1111/nph.16839
|
| |
|
| Contributor(s) |
Zhou Y, Gan X, Viñegra de la Torre N, Neumann U, Albani MC |
| Citation(s) |
32745288 |
| |
| Submission date |
Feb 21, 2020 |
| Last update date |
Jan 26, 2021 |
| Contact name |
Maria Albani |
| Organization name |
University of Cologne
|
| Department |
Botanical institute
|
| Street address |
Carl-von-Linné-Weg 10
|
| City |
Köln |
| ZIP/Postal code |
50829 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL23515 |
Illumina HiSeq 2500 (Arabis alpina) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE145718 |
APETALA2‐like transcription factor in shoot architecture and perennial traits |
|
| Relations |
| BioProject |
PRJNA608065 |
| SRA |
SRP250385 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE145716_converted_variant_V3.6.xlsx |
1.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
