|Public on Jan 26, 2021
|APETALA2‐like transcription factor in shoot architecture and perennial traits
|Expression profiling by high throughput sequencing
Genome variation profiling by high throughput sequencing
|Polycarpic perennials maintain vegetative growth after ﬂowering. PERPETUAL FLOWERING 1 (PEP1), the orthologue of FLOWERING LOCUS C (FLC) inArabis alpina regulates ﬂowering and contributes to polycarpy in a vernalisation-dependent pathway. pep1 mutants do not require vernalisation to ﬂower and have reduced return to vegetative growth as all of their axillary branches become reproductive.
To identify additional genes that regulate ﬂowering and contribute to perennial traits we performed an enhancer screen of pep1. Using mapping-by-sequencing, we cloned a mutant (enhancer of pep1-055, eop055), performed transcriptome analysis and physiologically characterised the role it plays on perennial traits in an introgression line carrying the eop055 mutation and a functional PEP1 wild-type allele.
eop055 ﬂowers earlier than pep1 and carries a lesion in the A. alpina orthologue of the APETALA2 (AP2)-like gene, TARGET OF EAT2 (AaTOE2). AaTOE2 is a ﬂoral repressor and acts upstream of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 5 (AaSPL5). In the wild-type background, which requires cold treatment to ﬂower, AaTOE2 regulates the age-dependent response to vernalisation. In addition, AaTOE2 ensures the maintenance of vegetative growth by delaying axillary meristem initiation and repressing ﬂowering of axillary buds before and during cold exposure.
We conclude that AaTOE2 is instrumental in ﬁne tuning different developmental traits in the perennial life cycle of A. alpina.
|Refer to individual Series
|http:// http:// https://doi.org/10.1111/nph.16839
|Feb 21, 2020
|Last update date
|Jan 26, 2021
|University of Cologne
|Illumina HiSeq 2500 (Arabis alpina)
|Illumina HiSeq 3000 (Arabis alpina)
|This SuperSeries is composed of the following SubSeries:
|Beyond flowering time: diverse roles of an APETALA2‐like transcription factor in shoot architecture and perennial traits
| Beyond flowering time: diverse roles of an APETALA2‐like transcription factor in shoot architecture and perennial traits