NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE145718 Query DataSets for GSE145718
Status Public on Jan 26, 2021
Title APETALA2‐like transcription factor in shoot architecture and perennial traits
Organism Arabis alpina
Experiment type Expression profiling by high throughput sequencing
Genome variation profiling by high throughput sequencing
Summary Polycarpic perennials maintain vegetative growth after flowering. PERPETUAL FLOWERING 1 (PEP1), the orthologue of FLOWERING LOCUS C (FLC) inArabis alpina regulates flowering and contributes to polycarpy in a vernalisation-dependent pathway. pep1 mutants do not require vernalisation to flower and have reduced return to vegetative growth as all of their axillary branches become reproductive.
To identify additional genes that regulate flowering and contribute to perennial traits we performed an enhancer screen of pep1. Using mapping-by-sequencing, we cloned a mutant (enhancer of pep1-055, eop055), performed transcriptome analysis and physiologically characterised the role it plays on perennial traits in an introgression line carrying the eop055 mutation and a functional PEP1 wild-type allele.
eop055 flowers earlier than pep1 and carries a lesion in the A. alpina orthologue of the APETALA2 (AP2)-like gene, TARGET OF EAT2 (AaTOE2). AaTOE2 is a floral repressor and acts upstream of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 5 (AaSPL5). In the wild-type background, which requires cold treatment to flower, AaTOE2 regulates the age-dependent response to vernalisation. In addition, AaTOE2 ensures the maintenance of vegetative growth by delaying axillary meristem initiation and repressing flowering of axillary buds before and during cold exposure.
We conclude that AaTOE2 is instrumental in fine tuning different developmental traits in the perennial life cycle of A. alpina.
 
Overall design Refer to individual Series
Web link http:// http:// https://doi.org/10.1111/nph.16839
 
Citation(s) 32745288
Submission date Feb 21, 2020
Last update date Jan 26, 2021
Contact name Maria Albani
Organization name University of Cologne
Department Botanical institute
Street address Carl-von-Linné-Weg 10
City Köln
ZIP/Postal code 50829
Country Germany
 
Platforms (2)
GPL23515 Illumina HiSeq 2500 (Arabis alpina)
GPL26216 Illumina HiSeq 3000 (Arabis alpina)
Samples (14)
GSM4331498 WT_shoot apex_rep1
GSM4331499 WT_shoot apex_rep2
GSM4331500 WT_shoot apex_rep3
This SuperSeries is composed of the following SubSeries:
GSE145715 Beyond flowering time: diverse roles of an APETALA2‐like transcription factor in shoot architecture and perennial traits
GSE145716 Beyond flowering time: diverse roles of an APETALA2‐like transcription factor in shoot architecture and perennial traits
Relations
BioProject PRJNA607995

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
SRA Run SelectorHelp

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap