GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE145909 Query DataSets for GSE145909
Status Public on Nov 23, 2021
Title Genome-wide Analysis of Sp6/Epiprofin DNA Binding Sites in the Developing Mouse Molar Using ChIP-seq Analysis
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In this study, whole-genome ChIP-seq analysis was performed to identify Sp6 regulatory targets from postnatal mouse molars. Enriched sequence reads (peaks) from Sp6 immunoprecipitations were located predominately adjacent to transcriptional start sites. Notably enriched for Sp6 peaks were genes highly-expressed in the tooth. Bioinformatic analysis of two pools of 1400 Sp6 enriched sequences identified a consensus nine-nucleotide DNA pulldown sequence of CTg/aTAATTA from mesenchyme and the nearly identical sequence, CTa/gTAATTA, from epithelium. Sp6 AT-rich interaction sequences are nearly identical to the ChIP-seq enriched sequences reported for Sp7 and Sp9. ChIP-seq analysis indicates Sp6 regulates a significant number of genes expressed in the tooth.

GSE145909 is the original submission. GSE151391 is also from the same study.
Overall design Analysis of Sp6 DNA binding in dental epithelium and dental mesenchyme using ChIP-seq analysis
Contributor(s) Yoshitomi Y, Rhodes CS, Freese NH, Nakamura T, Chiba Y, Yamada Y
Citation(s) 34662808
Submission date Feb 25, 2020
Last update date Nov 24, 2021
Contact name Yasuo Yoshitomi
Organization name NIH
Department NIDCR
Street address 30 Convent Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (4)
GSM4339188 Sp6-mesenchyme
GSM4339189 Sp6-epithelium
GSM4339190 Input-mesenchyme
BioProject PRJNA608714
SRA SRP250706

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE145909_RAW.tar 583.0 Mb (http)(custom) TAR (of BAR, BED, XLSX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap