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Series GSE14605 Query DataSets for GSE14605
Status Public on Feb 23, 2009
Title mRNA-Seq Whole Transcriptome Analysis of a Single Cell
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiDâ„¢ System to set up a single cell whole transcriptome assay. The modified amplification strategy allows us to amplify full-length cDNAs for most of the expressed genes. We show that it is feasible to get digital gene expression profiles at single cell resolution. This allows us to ask fundamental biological questions that could not be addressed previously, especially in the early embryonic development field, and to understand transcriptome complexity at the resolution of a single cell.
Overall design gene expression profiling from two single wild-type oocytes, two single Dicer knockout oocyte, and one single Ago2 knockout oocyte
Contributor(s) Tang F, Barbacioru C, Lao K, Surani MA
Citation(s) 19349980
Submission date Jan 27, 2009
Last update date Jun 11, 2013
Contact name Catalin Barbacioru
Organization name Applied Biosystems
Street address 850 Lincoln Centre Dr
City Foster City
State/province CA
ZIP/Postal code 94404
Country USA
Platforms (1)
GPL9318 AB SOLiD System 3.0 (Mus musculus)
Samples (6)
GSM365012 mouse 4-cell blastomer
GSM365013 mouse mature wild-type oocyte#1
GSM365014 mouse mature wild-type oocyte#2
BioProject PRJNA111725

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14605_RAW.tar 3.3 Gb (http)(custom) TAR (of SRF, TXT, WIG)
Processed data provided as supplementary file

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