|
| Status |
Public on Mar 12, 2020 |
| Title |
Lectin microarray analysis of murine CTL EVs separated by ion exchange method |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
EVs in culture supernatant can be concentrated with removing exsomeres and 97% free proteins by MWCO 750 kDa UF.
DEAE chromatography can be divided into bioactive EXO and other EVs as nucleic acid (DNA) cargo in UF-concentrated EVs.
|
| |
|
| Overall design |
Recently, instead of ultracentrifugation, development of new preparation protocol is demanded for research of reliable bioactivity and drug discovery of extracellular vesicles (EVs). In this study, we propose a novel method for large scale preparation of high-performance extracellular vesicles focusing on membrane negative charge. Murine cytotoxic T lymphocyte (CTL) EVs in supernatant are concentrated more than 20 times at over 97% purity without leaking by 750 kDa MWCO ultrafiltration, replaced with PBS, and subjected to ion exchange DEAE column chromatography.
|
| |
|
| Contributor(s) |
Seo N, Tateno H |
| Citation(s) |
35289089 |
| |
| Submission date |
Mar 11, 2020 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Naohiro Seo |
| E-mail(s) |
seo-naohiro@clin.medic.mie-u.ac.jp
|
| Organization name |
Mie University Graduate School of Medicine
|
| Department |
Immuno-Gene Therpy
|
| Street address |
2-174 Edobashi
|
| City |
Tsu |
| State/province |
Mie |
| ZIP/Postal code |
514-8507 |
| Country |
Japan |
| |
|
| Platforms (1) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA612028 |
Less...
More...