GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE146818 Query DataSets for GSE146818
Status Public on Mar 12, 2020
Title Lectin microarray analysis of murine CTL EVs separated by ion exchange method
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Expression profiling by array
Summary EVs in culture supernatant can be concentrated with removing exsomeres and 97% free proteins by MWCO 750 kDa UF.
DEAE chromatography can be divided into bioactive EXO and other EVs as nucleic acid (DNA) cargo in UF-concentrated EVs.
Overall design Recently, instead of ultracentrifugation, development of new preparation protocol is demanded for research of reliable bioactivity and drug discovery of extracellular vesicles (EVs). In this study, we propose a novel method for large scale preparation of high-performance extracellular vesicles focusing on membrane negative charge. Murine cytotoxic T lymphocyte (CTL) EVs in supernatant are concentrated more than 20 times at over 97% purity without leaking by 750 kDa MWCO ultrafiltration, replaced with PBS, and subjected to ion exchange DEAE column chromatography.
Contributor(s) Seo N, Tateno H
Citation(s) 35289089
Submission date Mar 11, 2020
Last update date Mar 31, 2022
Contact name Naohiro Seo
Organization name Mie University Graduate School of Medicine
Department Immuno-Gene Therpy
Street address 2-174 Edobashi
City Tsu
State/province Mie
ZIP/Postal code 514-8507
Country Japan
Platforms (1)
GPL28263 Rexxam(96 lectins) array chip
Samples (9)
GSM4407504 0.3 M Exo 1
GSM4407505 0.3 M Exo 2
GSM4407506 0.3 M Exo 3
BioProject PRJNA612028

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap