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Series GSE14687 Query DataSets for GSE14687
Status Public on Jul 22, 2009
Title Involvement of the brain renin-angiotensin system in the effects of maternal separation
Organism Mus musculus
Experiment type Expression profiling by array
Summary The postnatal development of the mouse is characterized by a stress hyporesponsive period (SHRP), where basal corticosterone levels are low and responsiveness to mild stressors is reduced. Maternal separation is able to disrupt the SHRP and is widely used to model early trauma. In this study we aimed at identifying of brain systems involved in acute and possible long-term effects of maternal separation. We conducted a microarray-based gene expression analysis in the hypothalamic paraventricular nucleus after maternal separation, which revealed 52 differently regulated genes compared to undisturbed controls, among them are 37 up-regulated and 15 down-regulated genes. One of the prominently unregulated genes, angiotensinogen, was validated using a in-situ hybridization. Angiotensinogen is the precursor of angiotensin II the main effector of the brain renin-angiotensin system (RAS), which is known to be involved in stress system modulation in adult animals. Using the selective angiotensin type I receptor (AT(1)) antagonist candesartan we found strong effects on CRH and GR mRNA expression in the brain and ACTH release following maternal separation. AT(1) receptor blockade appears to enhance central effects of maternal separation in the neonate, suggesting a suppressing function of brain RAS during the SHRP. Taken together, our results illustrate the molecular adaptations that occur in the paraventricular nucleus following maternal separation and identify signaling cascades, that control stress system activity in the neonate.

Keywords: phenotype
Overall design Litters were randomly assigned to either a maternally non-separated or 24 hour maternally separated condition. At the time of testing, all pups from a litter were sacrificed immediately by decapitation.
Contributor(s) Liebl C, Panhuysen M, Pütz B, Trümbach D, Deussing J, Wurst W, Müller MB, Schmidt MV
Citation(s) 19506703
Submission date Feb 03, 2009
Last update date Mar 20, 2012
Contact name Dietrich Trümbach
Organization name German Research Center for Environmental Health
Department Institute for Developmental Genetics
Street address Ingolstädter Landstraße 1
City München-Neuherberg
ZIP/Postal code 85764
Country Germany
Platforms (1)
Samples (10)
GSM366775 non-separated animals PVN, technical replicate 1
GSM366776 non-separated animals PVN, technical replicate 2
GSM366777 non-separated animals PVN, technical replicate 3
BioProject PRJNA111675

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE14687_RAW.tar 18.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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