GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE14708 Query DataSets for GSE14708
Status Public on Apr 01, 2009
Title Comparative analysis of gene expression in two yeast species and their interspecific hybrid
Platform organisms Saccharomyces cerevisiae; Saccharomyces paradoxus
Sample organisms Saccharomyces cerevisiae; Saccharomyces paradoxus; Saccharomyces cerevisiae x Saccharomyces paradoxus
Experiment type Expression profiling by array
Summary We compared the genome-wide expression profiles of two yeast species (S. cerevisiae and S. paradoxus) using a two-species microarray that contain species-specific probes and can thus measure the expression levels of the two species simultaneosly. In Addition, we used the array to measure expression levels of the interspecific hybrid of these yeast species, while discriminating between the alleles that correspond to the two parental species. Comparison of the between-species differences and the within-hybrid allele differences allows us to separate cis from trans effects. Also, comparison of the overall expression in the hybrids (both alleles) with their parental species allows us to analyze hybrid over-expression and under-expression.

Keywords: comparative transcriptome analysis, hybrid gene expression
Overall design We analyzed four conditions (rich media, glycerol, heat shock and TSA). For each conditions, we hybridized the pooled sample of both species dyed with cy3/cy5 and a sample of the hybrid dyed with the alternate fluorescent color. Each experiment was done with 2 biological repeats, except for the rich media experiments done with 4 biological repeats.
Contributor(s) Tirosh I, Reikhav S
Citation(s) 19407207
Submission date Feb 04, 2009
Last update date Sep 20, 2012
Contact name Itay Tirosh
Organization name Weizmann Institute of Science
Department Molecular genetics
Lab Naama Barkai
Street address herzl
City Rehovot
ZIP/Postal code 76100
Country Israel
Platforms (1)
GPL8157 Yeast two-species array for S.cerevisiae and S.paradoxus (2 X 105K)
Samples (20)
GSM367220 S.cerevisiae and S.paradoxus at rich media (SC) repeat #1 Cy3
GSM367221 S.cerevisiae and S.paradoxus at rich media (SC) repeat #2 Cy5
GSM367222 S.cerevisiae and S.paradoxus at rich media (SC) repeat #3 Cy3
BioProject PRJNA112093

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 128.1 Mb (ftp)(http) ZIP
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap